Proton-controlled synthesis of red-emitting carbon dots and application for hematin detection in human erythrocytes
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The Red-emitting nitrogen-doped carbon dots (N-CDs) are synthesized using o-phenylenediamine by a one-step method, and can serve as a fluorescent probe for “turn off” detection of hematin in human red cells. The red-emitting N-CDs can be obtained only in acidic conditions and the emission of the red-emitting N-CDs is pH-dependent, indicating proton-controlled synthesis and emission. The red-emitting N-CDs are 2.7 nm in mean size and have a uniform dispersion and exhibit a high quantum yield (12.8%) and great optical properties. The developed sensing system for hematin displays a linear response from 0.4 to 32 μM with a detection limit of 0.18 μM. Importantly, this fluorescent probe demonstrates a good potential practicability for the quantitative detection of hematin in complex matrixes.
KeywordsN-Doped carbon dots Red emission Proton-controlled synthesis Hematin detection Human erythrocytes
This study received financial support from the National Natural Science Foundation of China (No. 21675131) and the Innovation Foundation of Chongqing City for Postgraduate (CYB 16053).
Compliance with ethical standards
The study was approved by the Ethics Committee of Southwest University, and written informed consent was obtained from all individuals participating in the study prior to the collection of the human blood samples.
Conflict of interest
The authors declare that they have no competing interests.
- 11.Light WR, Olson JS. Transmembrane movement of heme. J Biol Chem. 1990;265:15623–31.Google Scholar
- 17.Kuross SA, Rank BH, Hebbel RP. Excess heme in sickle erythrocyte inside-out membranes: possible role in thiol oxidation. Blood. 1988;71:876–82.Google Scholar
- 24.Liu SC, Zhai S, Palek J. Detection of hemin release during hemoglobin S denaturation. Blood. 1988;71:1755–8.Google Scholar
- 29.Liu J, Li D, Zhang K, Yang M, Sun H, Yang B. One-step hydrothermal synthesis of nitrogen-doped conjugated carbonized polymer dots with 31% efficient red emission for in vivo imaging. Small. 2018:1703919.Google Scholar