Analytical and Bioanalytical Chemistry

, Volume 411, Issue 6, pp 1193–1202 | Cite as

A novel coating method for CE capillary using carboxymethyl-β-cyclodextrin-modified magnetic microparticles as stationary for electrochromatography enantioseparation

  • Xiaodong Sun
  • Jie Guo
  • Tao Yu
  • Yingxiang DuEmail author
  • Zijie Feng
  • Shiyuan Zhao
  • Zhifeng Huang
  • Jie Liu
Research Paper


Magnetic microparticles (MMPs) have been extensively studied and aroused considerable interest in separation science owing to their superior characteristics. In this paper, a novel coated capillary with carboxymethyl-β-cyclodextrin-functionalized magnetic microparticles (CD-MMPs) as stationary phase was constructed and then applied to establish an open-tubular capillary electrochromatography enantioseparation system. The preparation of the CD-MMP-coated open-tubular column was very convenient because the coating of the magnetic microparticles onto the capillary column could be easily manipulated by an external magnetic field. The preparation conditions of the coated capillary such as magnetic field intensity and coating time are discussed in detail. The new constructed CD-MMP capillary system was applied to separate enantiomers of several racemic drugs. Compared to the uncoated capillary system, obviously preferable separations of tested enantiomers were obtained. Several important parameters affecting the enantioseparation, such as CM-β-CD concentration, running buffer pH, organic solvent, and applied voltage, were systematically optimized. Furthermore, satisfactory repeatability and chemical stability of this new CD-MMP capillary system were achieved in the experiment.

Graphical abstract


Capillary electrochromatography Enantioseparation Carboxymethyl-β-cyclodextrin-functionalized magnetic microparticles Magnetic coating 









Carboxymethyl-β-cyclodextrin-modified APTES–Fe3O4 magnetic microparticles


Capillary electrophoresis


Capillary electrochromatography






N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride


Electroosmotic flow


Gas chromatography


High-performance liquid chromatography


Magnetic microparticles










Open-tubular capillary electrochromatography




Supercritical fluid chromatography





This work was supported by the Natural Science Foundation of Jiangsu Province (Program No.BK20150697) and the Project of the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Compliance with ethical standards

Conflict of interest

The authors have declared no conflict of interest.

Supplementary material

216_2018_1545_MOESM1_ESM.pdf (998 kb)
ESM 1 (PDF 997 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Analytical ChemistryChina Pharmaceutical UniversityNanjingChina
  2. 2.Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education)China Pharmaceutical UniversityNanjingChina
  3. 3.State Key Laboratory of Natural MedicinesChina Pharmaceutical UniversityNanjingChina

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