Analytical and Bioanalytical Chemistry

, Volume 411, Issue 5, pp 1107–1115 | Cite as

GO-amplified fluorescence polarization assay for high-sensitivity detection of aflatoxin B1 with low dosage aptamer probe

  • Hua Ye
  • Qianqian Lu
  • Nuo Duan
  • Zhouping WangEmail author
Research Paper


Aflatoxin B1 (AFB1) is the most toxic mycotoxin of the aflatoxins (AFs) and shows carcinogenic, teratogenic and mutagenic effects in humans and animals. AFB1 is widely seen in cereal products such as rice and wheat. This research proposed a low-cost, high-sensitivity fluorescence polarization (FP) assay for detection of AFB1 using aptamer biosensors based on graphene oxide (GO). The aptamers labelled with fluorescein amidite (FAM) were adsorbed on the surface of GO through π–π stacking and electrostatic interaction, thus forming aptamer/GO macromolecular complexes. Under these conditions, the local rotation of fluorophores was limited and the system had a high FP value. When there was AFB1 in the system, aptamers were dissociated from the GO surface and combined with AFB1 owing to their specificity to form aptamer/AFB1 complexes. As a result, large changes were observed in the molecular weights of aptamers before, and after, the combination, therefore leading to the apparent changes in FP value. The results showed that when only 10 nM of aptamer was used, the changes in FP and the AFB1 concentration had a favourable linear relationship within 0.05 to 5 nM of AFB1, and the lowest detection limit (LOD) was 0.05 nM. In addition, the recoveries of rice sample extract ranged from 89.2% to 112%. The method is simple, highly sensitive, cost-efficient and shows potential application prospects.


Aptamer Fluorescence polarization Graphene oxide Signal amplifier Aflatoxin B1 



This work was partly funded by the Science & Technology Support Program of Jiangsu Province [BE2017623], Jiangsu Agricultural Science and Technology Innovation Fund [CX(18)2025], the National Natural Science Fund of China [31871881], the Fundamental Research Funds for Central Universities [JUSRP51714B], the Distinguished Professor Program of Jiangsu Province and Jiangnan University Postgraduate Overseas Research Project.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human or animal subjects.

Supplementary material

216_2018_1540_MOESM1_ESM.pdf (218 kb)
ESM 1 (PDF 218 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Hua Ye
    • 1
    • 2
  • Qianqian Lu
    • 1
  • Nuo Duan
    • 1
  • Zhouping Wang
    • 1
    • 3
    • 4
    • 5
    Email author
  1. 1.State Key Laboratory of Food Science and Technology, School of Food Science and TechnologyJiangnan UniversityWuxiChina
  2. 2.College of Food EngineeringAnhui Science and Technology UniversityFengyangChina
  3. 3.National Engineering Research Center of Seafood, School of Food Science and TechnologyDalian Polytechnic UniversityDalianChina
  4. 4.International Joint Laboratory on Food SafetyJiangnan UniversityWuxiChina
  5. 5.Collaborative Innovation Center of Food Safety and Quality Control of Jiangsu ProvinceWuxiChina

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