High-throughput screening of toxic substances by extractive electrospray ionization mass spectrometry and their identification via databank construction
More than 200 toxic substances (including narcotic drugs, psychotropic drugs, organic phosphorus compounds, carbamates, pyrethroids and other pesticides, veterinary drugs, rodenticides, natural toxins, and other drugs) were identified and quantified using an ion-trap mass spectrometer. The advantages of this technique—its selectivity, accuracy, precision, utilization of only small amounts of the sample, and short analysis time for a single sample (less than 30s)—render it a rapid and accurate methodology for toxin screening. Subsequently, an extractive electrospray ionization (EESI) mass spectrometry database was established by combining the Xcalibur data processing system with NIST database software. This allowed unknown toxicants in urine and blood samples, stomach contents, and liver samples, as provided by the Jiangxi Provincial Public Security Department, to be analyzed and identified. This EESI methodology and databank has the potential for widespread application to the large-scale analysis of practical samples.
KeywordsToxicants EESI-MS Databank Screen
The work was supported by National Natural Science Foundation of China (nos. 21675021, 21607161), National Natural Science Foundation of China (No. 21520102007), Program for Changjiang Scholars and Innovative Research Team in Universities (PCSIRT) (no. IRT 13054). The Research Fund of East China University of Technology (no. DHBK2017117), National Natural Science Foundation of China (no. 21505017), and the Project of Jiangxi Provincial Department of Education (no. GJJ160544).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All individual participants who gave blood and urine samples read and approved this experiment.
The studies were approved by the ethics committee of Nan Chang University in accordance with guideline chapter 5, N.21.
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