High-performance thin-layer chromatography coupled with HPLC-DAD/HPLC-MS/MS for simultaneous determination of bisphenol A and nine brominated analogs in biological samples
Bisphenol A (BPA) and its brominated analogs exhibiting bioaccumulation potential, endocrine disruption, and reproductive toxicity have been worldwide detected in water, air, soil, and sediments. But few methods have been proposed for simultaneously determining a variety of these compounds in biological matrices, hindering the further study on their biological transformation/degradation and health risks. In this study, a simple, solvent-saving and sensitive method based on high-performance thin-layer chromatography (HPTLC) for sample pretreatment coupled with high-performance liquid chromatography-diode array detector (HPLC-DAD) (UV = 214 nm)/triple quadrupole mass spectrometry (MS/MS) was developed for determining BPA and its nine brominated analogs in biological samples. The method detection limits (MDLs) and method quantification limits (MQLs) for ten BPA analogs ranged from 0.8 to 685.7 ng g−1 dw (S/N = 3) and 2.7 to 2285.7 ng g−1 dw (S/N = 10), respectively. The recoveries were 64–124% with SD less than 10%. The RSD of intermediate precision was less than 11%, and matrix effects were lower than 19%. Compared with traditional purification procedures, HPTLC largely reduced the workload and procedures for complex biological sample cleanup without inducing decomposition of the analytes. The proposed method exhibited good performance when detecting these ten chemicals in chicken samples from a nearby yard of brominated flame retardant plants, indicating its great potential for investigating their environment level, behavior, and fate in organisms.
KeywordsBisphenol A Brominated analogs Biological matrices HPTLC Samples cleanup
This work was supported by the National Natural Science Foundation of China (21707148), the China Postdoctoral Science Foundation (2016M602210 and 2018T110715) and the Open Fund supported by the State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences (KF2016-12).
Compliance with ethical standards
All procedures performed in studies involving animals were in accordance with the ethical standards of the care and use of animals in china. The studies have been approved by the Academic Committee (Ethics Committee) of Qingdao Institute of Bioenergy and Bioprocess Technology, China Academy of Sciences.
Conflict of interest
The authors declare that they have no conflict of interest.
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