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Analytical and Bioanalytical Chemistry

, Volume 411, Issue 7, pp 1319–1330 | Cite as

Comparison of turn-on and ratiometric fluorescent G-quadruplex aptasensor approaches for the detection of ATP

  • Sathya Srinivasan
  • Velu Ranganathan
  • Maria C. DeRosaEmail author
  • Bhaskar Mohan MurariEmail author
Research Paper

Abstract

Two fluorescent aptasensor methods were developed for the detection of ATP in biochemical systems. The first method consisted of a label-free fluorescent “turn-on” approach using a guanine-rich ATP aptamer sequence and the DNA-binding agent berberine complex. In the presence of ATP, the ATP preferentially binds with its aptamer and conformationally changes into a G-quadruplex structure. The association of berberine with the G-quadruplex results in the enhancement of the fluorescence signal of the former. The detection limit of ATP was found to be 3.5 μM. Fluorescence, circular dichroism and melting temperature (Tm) experiments were carried out to confirm the binding specificity and structural changes. The second method employs the ratiometric fluorescent approach based on the Forster resonance energy transfer (FRET) for the detection of ATP using berberine along with a quencher (AuNRs, AgNPs) and a fluorophore (red quantum dots (RQDs), carbon dots (CDs)) labeled at 5′ and 3′ termini of the ATP-binding aptamer sequence. Upon addition of ATP and berberine, ATP specifically binds with its aptamer leading to the formation of G-quadruplex, and similarly, berberine also binds to the G-quadruplex. This leads to an enhancement of fluorescence of berberine while that of RQD and CDs were significantly quenched via FRET. The respective detection limits calculated were 3.6 μM and 3.8 μM, indicating these fluorescent aptasensor methods may be used for a wide variety of small molecules.

Graphical abstract

Keywords

Adenosine-5′-triphosphate FRET Gold nanorods Fluorescence Berberine Aptasensor 

Notes

Funding information

This work was financially supported by the Natural Sciences and Engineering Research Council of Canada.

Compliance with ethical standards

The human serum samples used were pooled human samples obtained under informed consent (Millipore). All experiments were performed in accordance with the policies of Carleton University.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2018_1484_MOESM1_ESM.pdf (1.5 mb)
ESM 1 (PDF 1554 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019
corrected publication 2019

Authors and Affiliations

  1. 1.Department of BiotechnologyVIT VelloreVelloreIndia
  2. 2.Department of ChemistryCarleton UniversityOttawaCanada
  3. 3.Department of Sensor and Biomedical Technology, School of Electronics EngineeringVIT VelloreVelloreIndia

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