Complementarity of molecular and elemental mass spectrometric imaging of Gadovist™ in mouse tissues
Drug biodistribution analyses can be considered a key issue in pharmaceutical discovery and development. Here, mass spectrometric imaging can be employed as a powerful tool to investigate distributions of drug compounds in biologically and medically relevant tissue sections. Both matrix-assisted laser desorption ionization–mass spectrometric imaging as molecular method and laser ablation inductively coupled plasma–mass spectrometric imaging as elemental detection method were applied to determine drug distributions in tissue thin sections. Several mouse organs including the heart, kidney, liver, and brain were analyzed with regard to distribution of Gadovist™, a gadolinium-based contrast agent already approved for clinical investigation. This work demonstrated the successful detection and localization of Gadovist™ in several organs. Furthermore, the results gave evidence that gadolinium-based contrast agents in general can be well analyzed by mass spectrometric imaging methods. In conclusion, the combined application of molecular and elemental mass spectrometry could complement each other and thus confirm analytical results or provide additional information.
KeywordsMatrix-assisted laser desorption ionization–mass spectrometry imaging (MALDI-MSI) Laser ablation inductively coupled plasma–mass spectrometry imaging (LA-ICP-MSI) Gadolinium-based contrast agents (GBCAs)
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All procedures were approved by the guidelines and regulations of the Federation of Laboratory Animal Science Associations (FELASA) and the local guidelines and provisions for implementation of the Animal Welfare Act. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
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