Analytical and Bioanalytical Chemistry

, Volume 411, Issue 9, pp 1887–1894 | Cite as

Disposable carbon nanotube scaffold films for fast and reliable assessment of total α1-acid glycoprotein in human serum using adsorptive transfer stripping square wave voltammetry

  • Tania Sierra
  • Silvia Dortez
  • María Cristina González
  • F. Javier Palomares
  • Agustin G. CrevillenEmail author
  • Alberto EscarpaEmail author
Research Paper
Part of the following topical collections:
  1. Nanoparticles for Bioanalysis


Alpha-1-acid glycoprotein (AGP) is a serum glycoprotein whose levels are increased two or three times during disease or injury. This makes it a potential biomarker for inflammatory bowel diseases and sepsis. Consequently, fast, simple, and cheap analytical methods for prognosis, diagnosis, and follow-up of these diseases are demanded. In this work, we propose a simple electrochemical approach based on carbon nanotubes scaffold films (CNSFs) for total AGP determination in serum samples. Firstly, AGP is labeled with an electrochemical tag (osmium(VI) complex), and then the total amount of AGP is quantified by adsorptive transfer stripping square wave voltammetry (AdTSWV). Multi-walled carbon nanotubes scaffold films (MWSFs) yielded the best analytical performance in terms of sensitivity with a good limit of detection of 0.6 mg L−1 for AGP determination in serum samples, in less than 20 min. A simplified AGP calibration and its sequential serum sample analysis strategy with good accuracy (81%) and excellent reproducibility (RSD < 1%) was additionally proposed to meet the point-of-care/needs requirements.

Graphical abstract

Multi-walled carbon nanotubes scaffold films for total AGP determination on disposable platforms integrating single-point calibration and sequential sample analysis.


Alpha-1-acid glycoprotein Human serum Ligand exchange method Osmium(VI) Electrochemical tag Carbon nanotube 



Adsorptive transfer stripping square wave voltammetry


Alpha-1-acid glycoprotein


Britton–Robinson buffer


Carbon nanomaterial


Carbon nanotube scaffold film


Carbon nanotubes


Multi-walled carbon nanotubes


Multi-walled scaffold film


Point-of-care testing




Screen-printed carbon electrode


Single-walled carbon nanotubes


Single-walled scaffold film




Funding information

This work has been financially supported by the NANOAVANSENS program from the Community of Madrid (S2013/MIT-3029), the Spanish Ministry of Economy, Industry and Competitiveness (CTQ2017-86441-C2-1-R), and the MINECO (MAT2016-80394-R). T.S. acknowledges the FPI fellowship from the University of Alcala.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

216_2018_1419_MOESM1_ESM.pdf (158 kb)
ESM 1 (PDF 157 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Tania Sierra
    • 1
    • 2
  • Silvia Dortez
    • 1
  • María Cristina González
    • 1
    • 2
  • F. Javier Palomares
    • 3
  • Agustin G. Crevillen
    • 4
    Email author
  • Alberto Escarpa
    • 1
    • 2
    Email author
  1. 1.Department of Analytical Chemistry, Physical Chemistry and Chemical EngineeringUniversity of AlcalaAlcalá de HenaresSpain
  2. 2.Chemical Research Institute “Andrés M. del Río” (IQAR)University of AlcaláAlcalá de HenaresSpain
  3. 3.Instituto de Ciencia de Materiales de Madrid, CSICMadridSpain
  4. 4.Department of Analytical Sciences, Faculty of SciencesUniversidad Nacional de Educación a Distancia (UNED)MadridSpain

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