Disposable amperometric immunosensor for Saccharomyces cerevisiae based on carboxylated graphene oxide-modified electrodes
A sensitive and disposable amperometric immunosensor for Saccharomyces cerevisiae was constructed by using carbon screen-printed electrodes modified with propionic acid-functionalized graphene oxide as transduction element. The affinity-based biosensing interface was assembled by covalent immobilization of a specific polyclonal antibody on the carboxylate-enriched electrode surface via a water-soluble carbodiimide/N-hydroxysuccinimide coupling approach. A concanavalin A-peroxidase conjugate was further used as signaling element. The immunosensor allowed the amperometric detection of the yeast in buffer solution and white wine samples in the range of 10–107 CFU/mL. This electroanalytical device also exhibited low detection limit and high selectivity, reproducibility, and storage stability. The immunosensor was successfully validated in spiked white wine samples.
KeywordsSaccharomyces cerevisiae Immunosensor Graphene Wine Screen-printed electrodes
Financial support from the Spanish Ministry of Economy and Competitiveness (MINECO Projects CTQ2014-58989-P, CTQ2015-71936-REDT and CTQ2017-87954-P), the Junta de Comunidades de Castilla La Mancha (JCCM Project POII-2014-011-A), and the Comunidad de Madrid, Programme NANOAVANSENS (Project S2013/MIT-3029) is gratefully acknowledged.
Compliance with ethical standards
Declaration of conflict of interest
The authors declare that they have no conflict of interest.
- 2.International Organization of Vine and Wine. (2018) State of the vitiviniculture world market. Available from: http://www.oiv.int Accessed 6 October 2017.
- 3.Fleet GH, Heard GM. Yeast-growth during fermentation. In: Fleet GH, editor. Wine microbiology and biotechnology. Boca Ratón: CRC Press; 1993. p. 27–54.Google Scholar
- 28.Eletxigerra U, Martínez-Perdiguero J, Merino S, Barderas R, Ruiz-Valdepeñas Montiel V, Villalonga R, et al. Electrochemical magnetoimmunosensor for progesterone receptor determination. Application to the simultaneous detection of estrogen and progesterone breast-cancer related receptors in raw cell lysates. Electroanalysis. 2016;28:1787–94.CrossRefGoogle Scholar
- 30.Esteban-Fernández de Ávila B, Araque E, Campuzano S, Pedrero M, Dalkiran B, Barderas R, et al. Dual functional graphene derivative-based electrochemical platforms for direct detection of TP53 gene with single nucleotide poly-morphism selectivity in different raw biological samples. Anal Chem. 2015;87:2290–8.CrossRefGoogle Scholar
- 34.Andorrà I, Monteiro M, Esteve-Zarzoso B, Albergaria H, Mas A. Analysis and direct quantification of Saccharomyces cerevisiae and Hanseniaspora guilliermondii populations during alcoholic fermentation by fluorescence in situ hybridization, flow cytometry and quantitative PCR. Food Microbiol. 2011;28:1483–91.CrossRefGoogle Scholar