Analytical and Bioanalytical Chemistry

, Volume 410, Issue 25, pp 6653–6661 | Cite as

Preparation of copper tetra(N-carbonylacrylic) aminephthalocyanine functionalized zwitterionic-polymer monolith for highly specific capture of glycopeptides

  • Wenjuan Zhang
  • Liyan Jiang
  • Dongze Wang
  • Qiong JiaEmail author
Research Paper


In this work, poly(glycidyl methacrylate-ethyleneglycol dimethacrylate) monolith functionalized with copper tetra(N-carbonylacrylic) aminephthalocyanine and iminodiacetic acid was successfully synthesized. Owing to hydrogen bonding and hydrophilic interactions, the monolith exhibited good performance for glycopeptide enrichment. When the tryptic digests of horseradish peroxidase were enriched by the developed monolith, a total of 20 glycopeptides could be captured and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis with a detection limit as low as 0.5 fmol μL−1. With the mixture of bovine serum albumin and horseradish peroxidase digests (200:1, m/m) as the sample, 14 glycopeptides were identified after enrichment, showing the high selectivity of the monolith. Moreover, the functionalized monolith exhibited good stability and reproducibility. It was successfully applied to enrich glycopeptides from human serum, demonstrating its potential applications in selective and efficient capture of glycopeptides in complex biological samples.

Graphical abstract


Monolith Phthalocyanine Enrichment Glycopeptide Mass spectrometry 



This project was supported by the National Natural Science Foundation of China (21575049).

Compliance with ethical standards

The use of serum samples in this experiment was approved by the Ethics Committee of the First Hospital of Jilin University (Changchun, China).

Conflict of interest

The authors declare that they have no conflict of interest.

Informed consent

Informed consent was obtained from all participants in the experiment.

Supplementary material

216_2018_1278_MOESM1_ESM.pdf (1.2 mb)
ESM 1 (PDF 1248 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Wenjuan Zhang
    • 1
  • Liyan Jiang
    • 2
  • Dongze Wang
    • 3
  • Qiong Jia
    • 1
    • 2
    Email author
  1. 1.College of ChemistryJilin UniversityJilinChina
  2. 2.Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life SciencesJilin UniversityJilinChina
  3. 3.School of Pharmaceutical SciencesJilin UniversityJilinChina

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