Analytical and Bioanalytical Chemistry

, Volume 410, Issue 22, pp 5545–5553 | Cite as

Multiclass screening of >200 pharmaceutical and other residues in aquatic foods by ultrahigh-performance liquid chromatography–quadrupole-Orbitrap mass spectrometry

  • Cong KongEmail author
  • Yang Wang
  • Yuanfei Huang
  • Huijuan YuEmail author
Research Paper
Part of the following topical collections:
  1. Food Safety Analysis


A quick screening method of more than 200 pharmaceutical and other residues in aquatic foods based on ultrahigh-performance liquid chromatography–quadrupole-Orbitrap mass spectrometry (UHPLC-Q/Orbitrap MS) was established. In this method, after the addition of 200 μL of 1 M EDTA-Na2, 2 g of each sample homogenate was extracted successively with 10 mL of acetonitrile and 10 mL of ethyl acetate. The extracts were combined, dried under nitrogen flow, and redissolved in 0.1% formic acid in acetonitrile/water (4:6, v/v) for analysis. The prepared samples were analyzed by UHPLC- Q/Orbitrap MS system in Full MS/ddMS2 (full-scan data-dependent MS/MS) mode. Compound identification was performed through comparison of the sample data with the database for standard chemicals, including the retention time, precursor ion, product ions, and isotope pattern for all 206 compounds. Five different aquatic food matrices (carp, shrimp, crab, eel, and mussel) spiked with the analytes at 1, 10, and 50 ng/g were evaluated to assess recoveries, precision, matrix effects, stability, and detection limits using the method. UHPLC analyses required 25 min, and 178–200 analytes met identification criteria at 50 ng/g depending on the matrix. Furthermore, practical application of this method for real samples displayed strong screening capability.

Graphical abstract

A quick screening method of >200 pharmaceutical and other residues in aquatic foods based on ultrahighperformance liquid chromatography–quadrupole-Orbitrap mass spectrometer was established. Fivedifferent aquatic food matrices, including carp, shrimp, crab, eel and mussel, were studied to evaluatescreen limit at 1, 10 and 50 μg·kg-1 level. Results suggest the high reliability, high time-efficiency and goodsimplicity of the method.


Veterinary drug Aquatic food Screening Orbitrap Pesticide Contaminant residue 



The authors would like to acknowledge the financial support from the Shanghai Municipal Agricultural Commission, the Yangfan project (14YF1408100) from Science and Technology Commission of Shanghai Municipality – PR China and the special research fund for the national non-profit institutes (East China Sea Fisheries Research Institute) (No. 2014T05).

Compliance with ethical standards

This research did not involve human participants or animals.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2018_1124_MOESM1_ESM.pdf (3.3 mb)
ESM 1 (PDF 3.29 MB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research InstituteChinese Academy of Fishery SciencesShanghaiChina
  2. 2.College of Food Science & TechnologyShanghai Ocean UniversityShanghaiChina
  3. 3.Key Laboratory of Oceanic and Polar Fisheries, Ministry of Agriculture, East China Sea Fisheries Research InstituteChinese Academy of Fishery SciencesShanghaiChina

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