Analysis of a variety of inorganic and organic additives in food products by ion-pairing liquid chromatography coupled to high-resolution mass spectrometry
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A reversed-phase ion-pairing chromatographic method was developed for the detection and quantification of inorganic and organic anionic food additives. A single-stage high-resolution mass spectrometer (orbitrap ion trap, Orbitrap) was used to detect the accurate masses of the unfragmented analyte ions. The developed ion-pairing chromatography method was based on a dibutylamine/hexafluoro-2-propanol buffer. Dibutylamine can be charged to serve as a chromatographic ion-pairing agent. This ensures sufficient retention of inorganic and organic anions. Yet, unlike quaternary amines, it can be de-charged in the electrospray to prevent the formation of neutral analyte ion-pairing agent adducts. This process is significantly facilitated by the added hexafluoro-2-propanol. This approach permits the sensitive detection and quantification of additives like nitrate and mono-, di-, and triphosphate as well as citric acid, a number of artificial sweeteners like cyclamate and aspartame, flavor enhancers like glutamate, and preservatives like sorbic acid. This is a major advantage, since the currently used analytical methods as utilized in food safety laboratories are only capable in monitoring a few compounds or a particular category of food additives.
KeywordsAnionic food additives Food safety Ion-pairing chromatography High-resolution mass spectrometry Orbitrap
Compliance with ethical standards
The authors adhered to ethical standards.
Conflict of interest
The authors declare that they have no conflicts of interest.
No studies involving human participants or animals were conducted.
- 1.Kubica P, Namiesnik J, Wasik A. Sweeteners and common Stevia rebaudiana glycosides in non-alcoholic and alcoholic beverages by reversed-phase liquid chromatography coupled with tandem mass spectrometry. J Anal Bioanal Chem. 2014;406:1505–12. https://doi.org/10.1007/s00216-014-8355-x.CrossRefGoogle Scholar
- 2.Lorenzo-Fernandez M, Lopez-Hernandez J, Pineiro-Sotelo M, Rodriguez-Bernaldo A. High performance liquid chromatography for joint determination of the flavour enhancers monosodium glutamate, disodium guanylate and disodium inosinate in dehydrated bouillons. Dtsch Lebensm Rundsch. 2002;98:93–895.Google Scholar
- 4.Goncalves AG, Rech TB, Rodrigues PM. Quality evaluation of frozen seafood (Genypterus brasiliensis, Prionotus punctatus, Pleoticus muelleri and Perna perna) previously treated with phosphates. Pan-Am J Aquat Sci. 2008;3(3):248–58.Google Scholar
- 9.Dafflon O, Scheurer O, Gobet H, Bossed J. Polyphosphate determination in seafood and processed cheese using high-performance anion-exchange chromatography after phosphatase inhibition using microwave heat shock. Mitt Lebensm Hyg. 2003;94:127–35.Google Scholar
- 11.Gilchrist E, Nesterenko P, Smith N, Barron L. Organic solvent and temperature-enhanced ion chromatography-high resolution mass spectrometry for the determination of low molecular weight organic and inorganic anions. Anal Chim Acta. 2015;865:83–91. https://doi.org/10.1016/j.aca.2015.01.031.CrossRefGoogle Scholar
- 12.Saccani C, Tanzi E, Cavalli S, Rohrer J. Determination of nitrite, nitrate, and glucose-6-phosphate in muscle tissues and cured meat by LC/MS. J AOAC. 2006;89:712–9.Google Scholar
- 13.Studzinska S, Rola R, Buszewski B. The impact of ion-pairing reagents on the selectivity and sensitivity in the analysis of modified oligonucleotides in serum samples by liquid chromatography coupled with tandem mass spectrometry. J Pharm Biomed Anal. 2017;138:146–52. https://doi.org/10.1016/j.pba.2017.02.014.CrossRefGoogle Scholar