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Analytical and Bioanalytical Chemistry

, Volume 410, Issue 22, pp 5629–5640 | Cite as

Analysis of a variety of inorganic and organic additives in food products by ion-pairing liquid chromatography coupled to high-resolution mass spectrometry

  • Anton Kaufmann
  • Mirjam Widmer
  • Kathryn Maden
  • Patrick Butcher
  • Stephan Walker
Research Paper
Part of the following topical collections:
  1. Food Safety Analysis

Abstract

A reversed-phase ion-pairing chromatographic method was developed for the detection and quantification of inorganic and organic anionic food additives. A single-stage high-resolution mass spectrometer (orbitrap ion trap, Orbitrap) was used to detect the accurate masses of the unfragmented analyte ions. The developed ion-pairing chromatography method was based on a dibutylamine/hexafluoro-2-propanol buffer. Dibutylamine can be charged to serve as a chromatographic ion-pairing agent. This ensures sufficient retention of inorganic and organic anions. Yet, unlike quaternary amines, it can be de-charged in the electrospray to prevent the formation of neutral analyte ion-pairing agent adducts. This process is significantly facilitated by the added hexafluoro-2-propanol. This approach permits the sensitive detection and quantification of additives like nitrate and mono-, di-, and triphosphate as well as citric acid, a number of artificial sweeteners like cyclamate and aspartame, flavor enhancers like glutamate, and preservatives like sorbic acid. This is a major advantage, since the currently used analytical methods as utilized in food safety laboratories are only capable in monitoring a few compounds or a particular category of food additives.

Graphical abstract

Deptotonation of ion pair agent in the electrospray interface

Keywords

Anionic food additives Food safety Ion-pairing chromatography High-resolution mass spectrometry Orbitrap 

Notes

Compliance with ethical standards

The authors adhered to ethical standards.

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

No studies involving human participants or animals were conducted.

Informed consent

Not applicable.

References

  1. 1.
    Kubica P, Namiesnik J, Wasik A. Sweeteners and common Stevia rebaudiana glycosides in non-alcoholic and alcoholic beverages by reversed-phase liquid chromatography coupled with tandem mass spectrometry. J Anal Bioanal Chem. 2014;406:1505–12.  https://doi.org/10.1007/s00216-014-8355-x.CrossRefGoogle Scholar
  2. 2.
    Lorenzo-Fernandez M, Lopez-Hernandez J, Pineiro-Sotelo M, Rodriguez-Bernaldo A. High performance liquid chromatography for joint determination of the flavour enhancers monosodium glutamate, disodium guanylate and disodium inosinate in dehydrated bouillons. Dtsch Lebensm Rundsch. 2002;98:93–895.Google Scholar
  3. 3.
    Chang CS, Yeh TS. Detection of 10 sweeteners in various foods by liquid chromatography/tandem mass spectrometry. J Food Drug Anal. 2014;22:318–28.  https://doi.org/10.1016/j.jfda.2014.01.024.CrossRefGoogle Scholar
  4. 4.
    Goncalves AG, Rech TB, Rodrigues PM. Quality evaluation of frozen seafood (Genypterus brasiliensis, Prionotus punctatus, Pleoticus muelleri and Perna perna) previously treated with phosphates. Pan-Am J Aquat Sci. 2008;3(3):248–58.Google Scholar
  5. 5.
    Ruiz-Calero V, Galceran MT. Ion chromatography separations of phosphorus species: a review. Talanta. 2005;66:376–410.  https://doi.org/10.1016/j.talanta.2005.01.027.CrossRefGoogle Scholar
  6. 6.
    Kaufmann A, Maden K, Leisser W, Gude T. Analysis of polyphosphates in fish and shrimps tissues by two different ion chromatographyy methods: implications on false-negative and positive findings. Food Addit Contam. 2005;22:1073–82.  https://doi.org/10.1080/02652030500239565.CrossRefGoogle Scholar
  7. 7.
    Baluyot E, Hartford C. Comparison of polyphosphate analysis by ion chromatography and by modified end-group titration. J Chrom A. 1996;739:217–22.CrossRefGoogle Scholar
  8. 8.
    Iammarino M, Di Taranto A. Determination of polyphosphates in products of animal origin: application of a validated ion chromatography method for commercial analyses. Eur Food Res Technol. 2012;235:409–17.CrossRefGoogle Scholar
  9. 9.
    Dafflon O, Scheurer O, Gobet H, Bossed J. Polyphosphate determination in seafood and processed cheese using high-performance anion-exchange chromatography after phosphatase inhibition using microwave heat shock. Mitt Lebensm Hyg. 2003;94:127–35.Google Scholar
  10. 10.
    Gallidabinio MD, Hamdan L, Murphy B, Barron L. Suspect screening of halogenated carboxylic acids in drinking water using ion exchange chromatography–high resolution (Orbitrap) mass spectrometry (IC-HRMS). Talanta. 2018;178:57–68.CrossRefGoogle Scholar
  11. 11.
    Gilchrist E, Nesterenko P, Smith N, Barron L. Organic solvent and temperature-enhanced ion chromatography-high resolution mass spectrometry for the determination of low molecular weight organic and inorganic anions. Anal Chim Acta. 2015;865:83–91.  https://doi.org/10.1016/j.aca.2015.01.031.CrossRefGoogle Scholar
  12. 12.
    Saccani C, Tanzi E, Cavalli S, Rohrer J. Determination of nitrite, nitrate, and glucose-6-phosphate in muscle tissues and cured meat by LC/MS. J AOAC. 2006;89:712–9.Google Scholar
  13. 13.
    Studzinska S, Rola R, Buszewski B. The impact of ion-pairing reagents on the selectivity and sensitivity in the analysis of modified oligonucleotides in serum samples by liquid chromatography coupled with tandem mass spectrometry. J Pharm Biomed Anal. 2017;138:146–52.  https://doi.org/10.1016/j.pba.2017.02.014.CrossRefGoogle Scholar
  14. 14.
    Basiri B, van Hattum H, van Dongen WD, Murph MM, Barlett MG. The rule of fluorinated alcohols as mobile phase modifiers for LC-MS analysis of oligonucleotides. J Am Soc Mass Spectr. 2017;28:190–9.  https://doi.org/10.1007/S13361-016-1500-3.CrossRefGoogle Scholar
  15. 15.
    Birdsall RE, Gilar M, Shion H, Yu YQ, Chen W. Reduction of metal adducts in oligonucleotide mass spectra in ion-pair reversed-phase chromatography/mass spectrometry analysis. Rapid Commun Mass Spectr. 2016;30:1667–79.  https://doi.org/10.1002/rcm.7596.CrossRefGoogle Scholar
  16. 16.
    Konieczka P, Namiesnik J. Estimation uncertainty in analytical procedures based on chromatographic techniques. J Chrom A. 2010;1217:882–91.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Anton Kaufmann
    • 1
  • Mirjam Widmer
    • 1
  • Kathryn Maden
    • 1
  • Patrick Butcher
    • 1
  • Stephan Walker
    • 1
  1. 1.Official Food Control AuthorityZürichSwitzerland

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