Analytical and Bioanalytical Chemistry

, Volume 411, Issue 7, pp 1409–1419 | Cite as

Development of a nitrogen-rich hyperbranched polymer as adsorbent for enrichment and determination of auxins in plants

  • Rui Zhang
  • Shanshan Li
  • Xiaoyan LiuEmail author
  • Haixia Zhang
Research Paper


In this study, a novel nitrogen-rich hyperbranched polymer was designed and synthesized via one-step precipitation copolymerization strategy. As possessing the lone-pair-electron-containing nitrogen atoms and positive-charged amine groups, as well as π electron–conjugated system, the prepared polymer displayed a strong tendency to adsorb protons acid, and negative-charged and conjugated compounds according to acid–base interaction, electrostatic interaction, and π–π stacking interaction. Based on these properties, a novel approach for assembling the proposed polymer coupled with high-performance liquid chromatography was successfully employed for selective enrichment and determination of auxins in plants. The extraction and desorption conditions were evaluated and the limits of detection and the limits of quantification of the proposed method were in the range of 0.15–0.29 μg L−1 and 0.49–0.98 μg L−1 for the four auxins based on the signal-to-noise ratio of 3:1 and 10:1, respectively. The recoveries of the target auxins from spiked plant samples were in the range from 85.0 to 116.3% with relative standard deviations lower than 9.6%. This study presented an inspiring thought for the construction of the versatile polymer adsorbent with highly efficient capturing of analytes from complex samples.

Graphical abstract


Hyperbranched polymer Solid-phase extraction Auxins High-performance liquid chromatography 


Funding information

The authors received support from the National Natural Science Foundation of China (NSFC) Fund (No. 21575055) and the Fundamental Research Funds for the Central Universities (lzujbky–2017–k09).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2018_1571_MOESM1_ESM.pdf (1.2 mb)
ESM 1 (PDF 1189 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Rui Zhang
    • 1
  • Shanshan Li
    • 1
  • Xiaoyan Liu
    • 1
    Email author
  • Haixia Zhang
    • 1
  1. 1.State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhouChina

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