Analytical and Bioanalytical Chemistry

, Volume 411, Issue 7, pp 1339–1349 | Cite as

A novel optical approach for determination of prolactin based on Pr-MOF nanofibers

  • Sheta M. ShetaEmail author
  • Said M. El-SheikhEmail author
  • Mokhles M. Abd-Elzaher
Research Paper


The analytical quantification and follow-up of the hormone prolactin is very important in clinical diagnosis (e.g., in cases of breast cancer), treatment, and the medical laboratory. The development of a new simple, fast, and less costly method is of considerable importance. Novel praseodymium metal–organic framework nanofibers (Pr-MOF-NFs) were synthesized by a facile and simple method for the determination of human prolactin in serum samples. The Pr-MOF-NFs were well characterized with several spectroscopic tools, such as mass spectrometry, Fourier transform IR spectroscopy, UV–vis spectroscopy, elemental analysis, X-ray diffraction, field-emission scanning electron microscopy combined with energy-dispersive X-ray spectroscopy, and high-resolution transmission electron microscopy. The photoluminescence of Pr-MOF-NFs was investigated, and the results revealed that Pr-MOF-NFs could be used as a sensitive and selective nanofiber optical sensor for the detection of human prolactin. The calibration graph was studied over a wide prolactin concentration range of 0–200 ng/mL, with limits of detection and quantitation of 0.276 and 0.838 ng/mL, respectively, lower than the values mentioned in previous reports. The correlation coefficient was 0.9792. Moreover, the Pr-MOF-NFs were applied successfully for the detection of serum human prolactin at clinically applicable concentrations without interference from several types of hormones and various interfering analytes.

Graphical abstract


Nanofibers Praseodymium Metal–organic frameworks Photoluminescence Optical approach Prolactin 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

The human serum samples used in this study were provided by the Family Medical Laboratory, Ministry of Health, Cairo, Egypt. Informed consent was obtained. The studies were approved by the appropriate ethics committee (Ministry of Health, Egypt) and were performed in accordance with ethical standards. We did not use the samples in any research involving human participants or research involving physical interventions on study participants or involving processing of personal data but conducted the research according to the method described in the article.

Supplementary material

216_2018_1564_MOESM1_ESM.pdf (757 kb)
ESM 1 (PDF 756 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Inorganic ChemistryNational Research CentreGizaEgypt
  2. 2.Department of Nanomaterials and NanotechnologyCentral Metallurgical R & D InstituteCairoEgypt

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