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Single-molecule tracking in eukaryotic cell nuclei

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Acknowledgements

U.K. gratefully acknowledges financial support by the Volkswagen Foundation (Grant I/78852) and the German Research Foundation (Grant Ku 1507/1-2).

Author information

Correspondence to Ulrich Kubitscheck.

Electronic supplementary material

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Single U1 snRNPs diffusing and binding within a living HeLa cell nucleus. Left hand side original but contrast-enhanced data. Right hand side movie data was filtered using a spot-enhancing filter (3-pixel diameter, see ref. [8]) to improve U1 snRNP visibility. The movie was recorded at 200 Hz, the total object field corresponded to an area of 12.2×12.2 μm2 (AVI 3.78 mb)

Movie 1

Single U1 snRNPs diffusing and binding within a living HeLa cell nucleus. Left hand side original but contrast-enhanced data. Right hand side movie data was filtered using a spot-enhancing filter (3-pixel diameter, see ref. [8]) to improve U1 snRNP visibility. The movie was recorded at 200 Hz, the total object field corresponded to an area of 12.2×12.2 μm2 (AVI 3.78 mb)

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Siebrasse, J.P., Grünwald, D. & Kubitscheck, U. Single-molecule tracking in eukaryotic cell nuclei. Anal Bioanal Chem 387, 41–44 (2007). https://doi.org/10.1007/s00216-006-0763-0

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Keywords

  • Splice Factor
  • Fluorescence Correlation Spectroscopy
  • Supramolecular Complex
  • Single Fluorescent Molecule
  • Small Nuclear Ribonucleoprotein Particle