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Combined ∆9-tetrahydrocannabinol and moderate alcohol administration: effects on ingestive behaviors in adolescent male rats

  • Nnamdi G. Nelson
  • Wen Xuan Law
  • Michael J. Weingarten
  • Lauren N. Carnevale
  • Aditi Das
  • Nu-Chu Liang
Original Investigation

Abstract

Rationale

Whereas co-use of alcohol and marijuana is prevalent in adolescents, the effects of such drug co-exposure on ingestive and cognitive behaviors remain largely unexplored. We hypothesized that co-exposure to alcohol and ∆9-tetrahydrocannabinol (THC), the main psychoactive constitute of marijuana, alters feeding behavior and cognition differently from either drug alone.

Methods

Male rats received daily THC (3–20 mg/kg/day) or oil vehicle through subcutaneous injection or consumption of a cookie with access to saccharin or saccharin-sweetened alcohol during adolescence (P30–45). Barnes maze and sucrose preference tests were applied to assess spatial memory and behavioral flexibility and abstinence-related anhedonia, respectively.

Results

Subcutaneous THC did not affect alcohol intake but dose-dependently increased acute (3 h) chow intake and reduced weight gain. Moderate alcohol consumption reduced the acute hyperphagic effect of subcutaneous THC. By contrast, oral THC at a dose > 5 mg/kg robustly reduced alcohol intake without affecting 3-h chow intake. At this dose, some rats stopped consuming the THC-laced cookies. Furthermore, oral THC reduced weight gain, and co-exposure to alcohol alleviated this effect. Chronic subcutaneous, but not oral, THC reduced sucrose intake during abstinence. Neither treatment impaired cognitive behaviors in the Barnes maze.

Conclusion

Moderate alcohol and THC consumption can interact to elicit unique outcomes on ingestive behaviors and energy balance. Importantly, this study established a novel model of voluntary alcohol and THC consumption for studying mechanisms underlying the consequences of adolescent onset co-use of the two drugs.

Keywords

Alcohol 9-tetrahydrocannabinol Adolescence Polydrug use Ingestive behavior Cognitive function Barnes maze Sucrose preference 

Notes

Acknowledgements

Startup funds from the Psychology Department of the University of Illinois at Urbana-Champaign (to NCL) and NIH grants R03DA043701 (to NCL), R01GM115584-01A1, and R03DA042365-01A1 (to AD) supported this study. We thank Dr. Lucas Li of the Roy J. Carver Metabolomics Center (UIUC) for performing the LC-MS/MS analyses. We acknowledge the technical assistance of Firmino Pinto, Charles Shoemaker, Jacqueline Sanchez, and Sheel Vasavada and are grateful to Dr. Justin Rhodes who provided access to the TopScan software. Portions of this work were presented at the 41st annual scientific meeting of the Research Society on Alcoholism in San Diego, CA, USA.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

213_2018_5093_MOESM1_ESM.docx (829 kb)
ESM 1 (DOCX 828 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Neuroscience ProgramUniversity of Illinois at Urbana-ChampaignChampaignUSA
  2. 2.Department of PsychologyUniversity of Illinois at Urbana-ChampaignChampaignUSA
  3. 3.Department of BiochemistryUniversity of Illinois at Urbana-ChampaignChampaignUSA
  4. 4.Department of Comparative BiosciencesUniversity of Illinois at Urbana-ChampaignChampaignUSA

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