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Aloin protects against arsenic trioxide–induced myocardial membrane damage and release of inflammatory cytokines

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Abstract

Aloin exerts concentration-dependent pro-oxidant and antioxidant effects when tested in vitro. Such duality of effects has not been investigated through in vivo studies on aloin. We evaluated the effects of aloin at doses ranging between 1 and 125 mg/kg against the arsenic trioxide (As2O3)–induced cardiotoxicity in mice. As2O3 (5 mg/kg/day) was intraperitoneally administrated for 10 days. Aloin was administered through oral gavage at 1, 5, 25, and 125 mg/kg/day. As2O3 induced rise in ST height and QT interval in ECG, increased oxidative stress, and depleted the antioxidative defense. As2O3 increased inflammatory cytokine concentrations in the heart. Aloin dose dependently inhibited the As2O3-induced cardiotoxicity. There was no evidence of increased oxidative stress in the low-dose aloin-treated mice receiving As2O3. Our results indicate that aloin possesses cardioprotective potentials and its pro-oxidant effect is not evident in vivo at tested doses.

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Author information

L. A. B., N. A. B., U. B. M. and C. R. P. carried out the literature search and conducted the animal studies, analyzed the results, and wrote the first draft of the manuscript. S. N. G. and S. O. helped in designing the study. D. D. P. carried out the analytical studies. S. B. and K. R. P. contributed to the design of the study and overall supervision. All authors read and approved the final manuscript.

Correspondence to Chandragouda R. Patil.

Ethics declarations

The study animals were maintained according to the guidelines of the Committee for the Purpose of Control and Supervision of the Experiments on Animals (CPCSEA) established under the Prevention of the Cruelty to Animals Act, 1960, Ministry of Environment and Forests, Government of India. The institutional animal ethics committee reviewed and sanctioned the experimental protocol (protocol approval no. IAEC/CPCSEA/RCPIPER/2018/19-03). The tests performed in this study complied with the protocols of the International Association for the Study of Pain.

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The authors declare that there is no conflict of interest.

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Birari, L.A., Mahajan, U.B., Patil, K.R. et al. Aloin protects against arsenic trioxide–induced myocardial membrane damage and release of inflammatory cytokines. Naunyn-Schmiedeberg's Arch Pharmacol (2020). https://doi.org/10.1007/s00210-020-01833-1

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Keywords

  • Aloin
  • As2O3
  • Oxidative stress
  • Myocardial damage
  • TNF-α