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NPPB prevents postoperative peritoneal adhesion formation by blocking volume-activated Cl current

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Abstract

5-Nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB) is a non-specific chloride channel blocker. Peritoneal adhesion is an inevitable complication of abdominal surgery and remains an important clinical problem, leading to chronic pain, intestinal obstruction, and female infertility. The aim of this study is to observe the effects of NPPB on peritoneal adhesions and uncover the underlying mechanism. The formation of postoperative peritoneal adhesions was induced by mechanical injury to the peritoneum of rats. MTT assay and wound-healing assay were used to evaluate proliferation and migration of primary cultured adhesion fibroblasts (AFB) respectively. Whole-cell chloride currents were measured using a fully automated patch-clamp workstation. Cell volume changes were monitored by light microscopy and video imaging. Our results demonstrated that NPPB could significantly prevent the formation of peritoneal adhesion in rats and inhibit the proliferation of AFB in a concentration-dependent manner. NPPB also reduced the migration of AFB cells with an IC50 of 53.09 μM. A 47% hypotonic solution successfully activated the ICl,vol in AFB cells. The current could be blocked by extracellular treatment with NPPB. Moreover, 100 μM NPPB almost completely eliminated the capacity of regulatory volume decrease (RVD) in these cells. These data indicate that NPPB could prevent the formation of postoperative peritoneal adhesions. The possible mechanism may be through the inhibition of the proliferation and migration of AFB cells by modulating ICl,vol and cell volume. These results suggest a potential clinical use of NPPB for preventing the formation of peritoneal adhesions.

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Funding

This work received funding from the Natural Science Foundation of Guangdong Province, China (2016A030313741), the Guangdong Provincial Thousand-Hundred-Ten Talent Project (2015cxqx214), and Guangzhou Science and Technology Project (201904010395)

Author information

B.X., J.M., and C.L. conceived and designed the research. J.Z., Z.Q., X.L., and J.S. conducted all experiments. B.X. and J.M. wrote the manuscript. All authors read and approved the manuscript.

Correspondence to Jianwen Mao or Bin Xu.

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All animal procedures in this work were conducted according to the Animal Subjects Ethics Subcommittee of Guangdong Pharmaceutical University, China.

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The authors declare that they have no conflict of interest.

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Zhong, J., Qin, Z., Yu, H. et al. NPPB prevents postoperative peritoneal adhesion formation by blocking volume-activated Cl current. Naunyn-Schmiedeberg's Arch Pharmacol 393, 501–510 (2020). https://doi.org/10.1007/s00210-019-01740-0

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Keywords

  • NPPB
  • Peritoneal adhesion
  • Chloride channel
  • Electrophysiology