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Effects of the solid lipid nanoparticle of carvacrol on rodents with lung injury from smoke inhalation

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The aim of this present study was to evaluate the effect of solid lipid nanoparticles (SLN) containing carvacrol over the lung damage of airway smoke inhalation. The study was conducted with 30 rats subjected to smoke inhalation and divided into 5 groups such as, normal control, negative control, oxygen group, SLN alone, and SLN+CARV group. The animals were sacrificed 24 h after the induction of inhalation injury further, the tissues of larynx, trachea, and lungs were collected for the histological, hematological, myeloperoxidase, and malondialdehyde analysis. The obtained results showed that treatment with CARV+SLN minimized the inhalation injury, since it reduced malondialdehyde significantly, when compared to the negative control group and minimized the histological changes which proves the absence of pulmonary emphysema and exudate in laryngeal and tracheal lumen in the CARV+SLN-treated group. Meanwhile, the presence of lesion with chronic characteristics was observed in the negative control and oxygen groups. It is suggested that the SLN containing carvacrol minimized oxidative stress and histological damages generated from smoke inhalation in rodents.

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This study received financial support from the Conselho Nacional de Desenvolvimento Científico e Tecnológico/CNPq/Brazil (Pronex 2014-Fapergs/CNPq) and Fundação de Amparo à Pesquisa do Estado de Sergipe/FAPITEC-SE.

Author information

FOC, ÉRS, FAF, KPPR, LASF, and VNBL conducted all the experimental procedures. FOC and SS wrote the whole manuscript. TVCO conducted the quantification of histological results. ADCS and SSG conducted the in vivo antioxidant studies. EAC provided the laboratory facility for the biochemical analysis. RLCAJ did the histopathological analysis. PSN, WLJ, LJQJ, and AASA conceived and designed the research.

Correspondence to Paula S. Nunes or Saravanan Shanmugam.

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All animal procedures were processed in accordance with the Normative Resolutions of the Conselho Nacional de Controle de Experimentação Animal - the Brazil’s CONCEA (CEPA # 011014).

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The authors declare that there is no conflict of interest.

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Carvalho, F.O., Silva, É.R., Nunes, P.S. et al. Effects of the solid lipid nanoparticle of carvacrol on rodents with lung injury from smoke inhalation. Naunyn-Schmiedeberg's Arch Pharmacol 393, 445–455 (2020). https://doi.org/10.1007/s00210-019-01731-1

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  • Lung injury
  • Carvacrol
  • Natural product
  • Inhalation
  • Smoke
  • Solid lipid nanoparticle