Protective effects of protopanaxatriol on acute liver injury induced by concanavalin A
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The purpose of this study was to explore the protective effect of protopanaxatriol (PPT) on acute liver injury induced by concanavalin A (ConA). In this study, mice were randomly separated into four groups. The first group received PBS (i.v.). The second group was given PPT (50 mg/kg body weight, i.p.) for 3 days before PBS (i.v.) injection. The third group received ConA (15 mg/kg body weight, i.v.). The fourth group was administered PPT (50 mg/kg body weight, i.p.) for 3 days before ConA (i.v.) injection. The serum levels of ALT and AST were detected after 20 h of ConA injection. The pathological changes of liver were observed by H/E staining. The expression of inflammatory factors was measured by ELISA and qRTPCR, and the changes of the signaling pathway were detected by western blot. Histopathological changes and blood transaminase elevation indicated significant liver injury after ConA injection. However, PPT pretreatment obviously reversed these changes. The ELISA and qRT-PCR results indicated that PPT preconditioning significantly inhibited the production of inflammatory factors. In addition, this inhibitory effect of PPT was mainly mediated by regulation of the nuclear factor-κB (NF-κB) signaling pathway. The active ingredient of ginseng, PPT, exerts an obvious protective effect on acute liver injury caused by ConA through inhibiting the inflammatory response.
KeywordsProtopanaxitriol ConA Liver injury NF-κB
Alanine aminotransferase (ALT)
Aspartate aminotransferase (AST)
Hematoxylin and eosin staining
Phosphate buffer saline
Tumor necrosis factor-alpha
G.N., Z.Z., and L.J. conceived the project and designed the study; L.J. and X.F. carried out all experiments and participated in the experimental design. L.J., J.Y., and S.Y. participated in the animal study. Z.Z. gave comments in experimental design and manuscript. G.N., Z.Z., and L.J. carried out data interpretation and discussion. L.J., X.F., and Z.Z. wrote the paper. All authors prepared and approved the paper for submission.
This study was supported by Shanghai Municipal Planning Commission of Science and Research Foundation (20134429, 20144Y0252), Shanghai University Youth Teacher Training program (ZZjdyx14005). This study was supported by Shanghai Municipal Natural Science Foundation (18ZR1433000). This study was supported by the National Natural Science Foundation of China (No. 81570719, 81670797 81471074, 81770863).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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