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Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 391, Issue 11, pp 1257–1274 | Cite as

Glibenclamide protects against thioacetamide-induced hepatic damage in Wistar rat: investigation on NLRP3, MMP-2, and stellate cell activation

  • Durgesh Kumar Dwivedi
  • G. B. Jena
Original Article

Abstract

Glibenclamide (GLB), most widely used in the treatment of type II diabetes mellitus, inhibits K+ATP channel in pancreatic-β cells and releases insulin, while thioacetamide (TAA) is a well-known hepatotoxicant and most recommended for the induction of acute and chronic liver disease. The purpose of this study was to evaluate the hepatoprotective potential of GLB against TAA-induced hepatic damage in Wistar rats. TAA (200 mg/kg, ip, twice weekly) and GLB (1.25, 2.5, and 5 mg/kg/day, po) were administered for 6 consecutive weeks. Different biochemical, DNA damage, histopathological, TEM, immunohistochemical, and western blotting parameters were evaluated. GLB treatment has no effects on the TAA-induced significant decrease in body and liver weights. TAA treatment significantly increased liver index and treatment with GLB has no effect the same. TAA treatment altered the liver morphology, whereas treatment with GLB normalized the alteration in morphology. Further, significant increase in oxidative stress, apoptosis, and DNA damage was found in TAA-treated animals and GLB treatment significantly reduced these effects. TAA-induced plasma transaminases and serum ALP levels were significantly restored by GLB. Furthermore, histopathological findings showed the presence of lymphocyte infiltration, collagen deposition, bridging fibrosis, degeneration of portal triad, and necrosis in TAA-treated animals and GLB intervention significantly reduced the same. TEM images revealed that GLB significantly normalized the hepatic stellate cell morphology as well as restored the number of lipid droplets. GLB treatment significantly downregulated the expressions of TGF-β1, α-SMA, NLRP3, ASC, caspase-1, and IL-1β, and upregulated MMP-2 and catalase against TAA-induced liver damage. The outcomes of the present study confirmed that GLB ameliorated the liver damage induced by TAA.

Keywords

Glibenclamide Thioacetamide Hepatic stellate cell Liver fibrosis NLRP3 

Abbreviations

ALP

Alkaline phosphatase

ALT

Alanine transaminase

ASC

Apoptosis-associated speck-like protein containing a caspase-recruiting domain

AST

Aspartate transaminase

ATP

Adenosine triphosphate

CYP2E1

Cytochrome P450 2E1

DAB

3,3′-Diaminobenzidine

DMSO

Dimethyl sulfoxide

ECM

Extracellular matrix

GLB

Glibenclamide

HSC

Hepatic stellate cell

IL

Interleukin

IL-1β

Interleukin-1 beta

ip

Intraperitoneal

MT

Masson’s trichrome

MMP-2

Matrix metalloproteinase-2

NLRP3

NACHT, LRR, and PYD domains-containing protein 3

TMO

Tail moment olive

PAS

Periodic acid-Schiff base

po

Per os (oral administration)

PSR

Picro Sirius Red

PSRF

PSR with fast green FCF

SOD

Superoxide dismutase

T2DM

Type II diabetes mellitus

TAA

Thioacetamide

TEM

Transmission electron microscopy

TGF-β1

Transforming growth factor beta1

TL

Tail length

TM

Tail moment

TUNEL

Terminal deoxynucleotidyl transferase dUTP nick end labeling assay

US-FDA

United States Food and Drug Administration

WHO

World Health Organization

α-SMA

Alpha-smooth muscle actin

γ-GT

γ-Glutamyl transferase

Notes

Acknowledgements

We wish to acknowledge the financial assistance received from National Institute of Pharmaceutical Education and Research (NIPER), S.A.S. Nagar, India, for carrying out the above experimentation. The authors would like to acknowledge Mr. Vinod Kumar for TEM image analysis. We are also grateful to Dr. Sabbir Khan, Dr. Krishna Prahlad Maremanda, and Dr. Venkateswara Rao Amara for their timely advice and technical support.

Authors’ contribution

DKD conceived, conducted the experiments, analyzed the data, and wrote the manuscript. GBJ conceived the idea, reviewed the manuscript, and administered the project. Finally, both the authors have read and approved the manuscript.

Compliance with ethical standards

The experimental protocol was approved by the Institutional Animal Ethics Committee (IAEC) against approval number IAEC/16/54.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

210_2018_1540_MOESM1_ESM.docx (16.9 mb)
ESM 1 (DOCX 17354 kb)

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© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Facility for Risk Assessment and Intervention Studies, Department of Pharmacology and ToxicologyNational Institute of Pharmaceutical Education and ResearchNagarIndia

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