Chavibetol corrects thyrotoxicosis through alterations in thyroid peroxidase
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Thyrotoxicosis is a clinical syndrome that commonly results from excess secretion and/or release of thyroid hormones in the circulation. It affects most of the body systems and if not treated properly may lead to serious health problems. In this investigation, we isolated a phenolic compound, chavibetol (CHV) from Piper betel leaf and evaluated its possible ameliorative effects in thyrotoxicosis of rats. Adult female rats were rendered thyrotoxic by the administration of l-thyroxine (l-T4) at 500 μg/kg/day, i.p., for 12 days, and then chavibetol (20.0 mg/kg, p.o.) was administered for 2 weeks. l-T4 administration elevated the concentration of serum thyroxine and triiodothyronine, activities of alanineaminotransferase and aspartate aminotransferase, and decreased the thyrotropin level as well as the expression of thyroid peroxidase (TPO). Further, it increased the activities of hepatic 5′mono-deiodinase-I, glucose-6--phosphatase, sodium-potasium-ATPase, and lipid peroxidation, and depleted the cellular antioxidants. However, chavibetol treatment to thyrotoxic rats normalized almost all these indices including TPO and also preserved the integrity of thyroid tissues suggesting its potential to correct thyrotoxicosis. Effects of CHV were more or less similar to a conventional antithyroid drug, propylthiouracil (PTU).
KeywordsChavibetol Thyrotoxicosis Antioxidants Thyroid peroxidase Lipid peroxidation 5′ Mono-deiodinase-I
FTIR, GC-MS, and NMR studies were made using the SAIF facilities from IIT, Chennai, India. We also thank Mr. Arif Khan for assisting in preparation of some figures.
Statement on the contributions of authors
S. panda and A. Kar conceived and designed the experiments. SP conducted the experiments, and R. Sharma contributed in statistical and data analyses. SP also wrote the manuscript, and A Kar revised and edited the same. All authors read and approved the manuscript for publication.
This work was supported by the grant received from Department of Science and Technology (DST), Govt. of India, New Delhi, India, under Women Scientist scheme to Dr. S. Panda [REF: SR/WOS-A/LS-407].
Compliance with ethical standards
The experimental protocol was approved by the Institutional Animal Ethical Committee (IAEC), registered with the Ministry of Social Justice and Empowerment, Government of India (registration No. 779/ Po/ Ere /S /03/ CPCSEA).
Conflict of interest
The authors declare that they have no conflicts of interest.
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