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Archives of Toxicology

, Volume 93, Issue 3, pp 727–741 | Cite as

Effects of diisononyl phthalate (DiNP) on the endocannabinoid and reproductive systems of male gilthead sea bream (Sparus aurata) during the spawning season

  • Isabel Forner-Piquer
  • Constantinos C. Mylonas
  • Ioannis Fakriadis
  • Maria Papadaki
  • Fabiana Piscitelli
  • Vincenzo Di Marzo
  • Josep Calduch-Giner
  • Jaume Pérez-Sánchez
  • Oliana CarnevaliEmail author
Reproductive Toxicology

Abstract

Diisononyl phthalate (DiNP) is a plasticizer used to improve plastic performance in a large variety of items which has been reported as an endocrine-disrupting chemical (EDC) in several organisms. The endocannabinoid system (ECS) is a cellular signaling system, whose functionality is tightly involved with reproductive function. The aim of the present study was the assessment of the effects of DiNP on the gonadal ECS and on the reproductive function of male gilthead sea bream Sparus aurata, an important marine aquacultured species in Europe, during the reproductive season. Fish were fed for 21 days with two diets contaminated with different nominal concentrations of DiNP (DiNP LOW at 15 µg DiNP kg−1 bw day−1 and DiNP HIGH at 1500 µg DiNP kg−1 bw day−1), based on the tolerable daily intake (TDI) ruled by the European Food Safety Authority for humans. The transcription of several genes related to the ECS was affected by the DiNP. Specifically, DiNP reduced the levels of endocannabinoids and endocannabinoid-like mediators, concomitant with the increase of fatty acid amide hydrolase (FAAH) activity. At the histological level, DiNP LOW induced the highest occurrence of individuals with regressed testes. Steroidogenesis was affected significantly, since plasma 11-ketotestosterone (11-KT), the main active androgen in fish, was significantly decreased by the DiNP HIGH treatment, while plasma 17β-estradiol (E2) levels were raised, associated with an increase of the gonadosomatic index (GSI). Additionally, the level of testosterone (T) was significantly increased in the DiNP LOW group, however, the same DiNP concentration reduced the levels of 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P). The production of sperm was in general not affected, since spermiation index, sperm density, survival and the duration of forward motility did not exhibit any changes compared to controls. However, computer-assisted sperm analysis (CASA) showed that DiNP reduced the percentage of motile cells. The results clearly suggest a negative effect of DiNP via the diet on the male endocrine system of gilthead sea bream during the reproductive season.

Keywords

DiNP Endocannabinoids Gonads Gilthead sea bream 

Abbreviations

DiNP

Diisononyl phthalate

ECS

Endocannabinoid system

EDC

Endocrine disruptor chemical

E2

17β-Estradiol

T

Testosterone

11-KT

11-Ketotestosterone

MIS

Maturation-inducing steroid or 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P)

AEA

Anandamide

2-AG

2-Arachidonoylglycerol

PEA

N-palmitoyl–ethanolamide

OEA

N-oleoyl-ethanolamide

CB1/cnr1

Endocannabinoid receptor type I

CB2/cnr2

Endocannabinoid receptor type II

TRPV1

Transient receptor potential cation channel subfamily V member 1

PPAR

Peroxisome proliferator-activated receptor

NAPE-PLD

N-acyl phosphatidylethanolamine phospholipase D

FAAH

Fatty acid amide hydrolase

DAGLα

Diacylglycerol lipase alpha

COX-2

Cyclooxigenase-2

ABDH

α/β-Hydrolase

CYT-PLA2

Cytosolic phospholipase A2

LEPR

Leptin receptor

LEPA

Leptin A

ER

Estrogen receptor

PR

Progesterone receptor

AR

Androgen receptor

LHR

Luteinizing hormone receptor

FSHR

Follicle-stimulating hormone receptor

GnRHR

Gonadotropin-releasing hormone receptor

17β-HSD

17β-Hydroxysteroid dehydrogenase

3β-HSD

3β-Hydroxysteroid dehydrogenase

VTGA

Vitellogenin A

ZP

Zona pellucida proteins

VCL

Curvilinear velocity

VSL

Straight line velocity

LIN

Linearity

VAP

Average path velocity

BW

Body weight

Notes

Acknowledgements

This work has received funding from Progetti di Rilevante Interesse Nazionale (PRIN) 2010–2011 (prot 2010W87LBJ) to OC and from the European Union’s Horizon 2020 research and innovation program under the TNA programme (Project ID AE030014) within AQUAEXCEL2020 project (652831) to IFP and OC for accessing to IATS-CSIC facilities. This output reflects only the author’s view and the European Union cannot be held responsible for any use that may be made of the information contained therein. The authors would like to thank the assistance of Marco Allarà for the enzymatic activity assay, Roberta Verde for assistance with the LC–MS/MS analyses and M. Ángeles González for technical assistance in PCR assays.

Compliance with ethical standards

Conflict of interest

The author(s) declare that they have no competing interests.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Isabel Forner-Piquer
    • 1
  • Constantinos C. Mylonas
    • 2
  • Ioannis Fakriadis
    • 2
  • Maria Papadaki
    • 2
  • Fabiana Piscitelli
    • 3
  • Vincenzo Di Marzo
    • 3
    • 5
  • Josep Calduch-Giner
    • 4
  • Jaume Pérez-Sánchez
    • 4
  • Oliana Carnevali
    • 1
    Email author
  1. 1.Dipartimento Scienze Della Vita e dell’AmbienteUniversità Politecnica Delle MarcheAnconaItaly
  2. 2.Institute of Marine Biology, Biotechnology and AquacultureHellenic Center for Marine ResearchHeraklionGreece
  3. 3.Endocannabinoid Research Group, Istituto di Chimica BiomolecolareConsiglio Nazionale Delle RicerchePozzuoliItaly
  4. 4.Nutrigenomics and Fish Endocrinology GroupInstitute of Aquaculture Torre de la Sal (IATS-CSIC)Ribera de CabanesSpain
  5. 5.Canada Excellence Research Chair on the Microbiome-Endocannabinoidome Axis in Metabolic HealthUniversité LavalQuebec CityCanada

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