Mucilaginibacter hurinus sp. nov., isolated from briquette warehouse soil
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A novel bacterial strain, designated ZR32T, was isolated from briquette warehouse soil in Ulsan (Korea). The strain was aerobic, showing pink-colored colonies on R2A agar. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ZR32T was closely related to Mucilaginibacter soli R9-65T (97.0%), Mucilaginibacter gynuensis YC7003T (96.9%), and Mucilaginibacter lutimaris BR-3T (96.8%). The values of DNA–DNA relatedness related two highest strains M. soli R9-65T and M. gynuensis YC7003T were 31.2 ± 6.9% and 19.7 ± 0.3%, respectively. Its genome size was 3.9 Mb, comprising 3402 predicted genes. The DNA G+C content of strain ZR32T was 43.0 mol%. The major cellular fatty acids (> 5% of total) were summed feature 3 (C16:1ω6c and/or C16:1ω7c), C16:0, C16:1ω5c, iso-C15:0, iso-C17:0 3-OH, and C17:1ω9c. The major respiratory quinine was menaquinone-7 (MK-7). The major polar lipids were phosphatidylethanolamine, two unidentified phospholipids, one unidentified sphingolipid, and one unidentified polar lipid. Strain ZR32T showed distinctive characteristics such as the temperature and pH for growth ranges, being positive for β-glucosidase, salicin production, negative for N-acetyl-glucosamine assimilation, being resistant to carbenicillin and piperacillin to related species. On the basis of phenotypic, chemotaxonomic, and phylogenetic data, strain ZR32T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter hurinus sp. nov. is proposed. The type strain is ZR32T (= KCTC 62193 = CCTCC AB 2017285).
KeywordsMucilaginibacter hurinus Polyphasic taxonomy
Korean Collection for Type Cultures
Agricultural Research Service Culture Collection
China Center for Type Culture Collection
Korean Agricultural Culture Collection
Transmission electron microscopy
National Center for Biotechnology Information
Trypticase soy agar
Molecular evolutionary genetics analysis
Guangdong Detection Center of Microbiology
This work was supported by the Development Program of China (National Development and Reform Commission; 2016YFD0800702). The authors are grateful to Prof. Dr. Bernhard Schink for the Latin construction of the species name. We acknowledge the Guangdong Detection Center of Microbiology (GDDCM) for fatty acid composition, polar lipids, and the DNA–DNA hybridization analyses. In addition, Genome was analyzed at Wuhan Frasergen Bioinformatics Co., Ltd.
LC: she has brought soil sample from Korea. She supported the procedures for the characterization for the strain ZR32T and the closest type strains. She conducted the PCR and sequencing of the 16S rRNA gene. She had constructed the phylogenetic trees, and Gram staining of the cells. She determined the cellular fatty acids. She had prepared the lyophilized powder of strain ZR32T and the closest related strains for analysis of polar lipids, isoprenoid quinone and DNA–DNA hybridization. She had conducted the experiments for bacterial phenotypic characteristics using the API strips. She had performed the analysis of the genome of ZR32T. She also made the tables and figure and wrote the manuscript. XZ: She helped Ms. Choi to collect colonies. In addition, she had conducted the experiments for bacterial phenotypic characteristics (growth temperature test, growth pH test, growth NaCl test, and motility test). YS: she supported the whole methods for the characterization for the strain ZR32T and the closest type strains. MW: he helped to correct grammar errors. In addition, he helped to revise the paper. GW: she supported the funding for the study. In addition, she advised about the way of experiments. ML: she proposed strain ZR32T as a new species. In addition, she advised about experimental skills.
Compliance with ethical standards
Conflict of interest
There is no conflict of interest.
- Bernardet JF, Nakagawa Y, Holmes B, Subcommittee on the taxonomy of Flavobacterium, and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes (2002) Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070. https://doi.org/10.1099/00207713-52-3-1049 Google Scholar
- Cappuccino JG, Sherman N (2002) Microbiology: a laboratory manual, 6th edn. Benjamin/Cummings, Menlo ParkGoogle Scholar
- Chen XY, Zhao R, Tian Y, Kong BH, Li XD et al (2014) Mucilaginibacter polytrichastri sp. nov., isolated from a moss (Polytrichastrum formosum), and emended description of the genus Mucilaginibacter. Int J Syst Evol Microbiol 64:1395–1400. https://doi.org/10.1099/ijs.0.055335-0 CrossRefGoogle Scholar
- Collins MD, Jones D (1980) Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470. https://doi.org/10.1111/j.1365-2672.1980.tb01036.x CrossRefGoogle Scholar
- Dong XZ, Cai MY (2001) Determinative manual for routine bacteriology. Scientific Press, BeijingGoogle Scholar
- Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229. https://doi.org/10.1099/00207713-39-3-224 CrossRefGoogle Scholar
- Kroppenstedt RM (1985) Fatty acid and menaquinone analysis of actinomycetes and related organisms. In: Goodfellow M, Minnikin DE (eds) Chemical methods in bacterial systematics. Academic Press, pp 173–199. https://www.mendeley.com/research-papers/fatty-acid-menaquinone-analysis-actinomycetes-related-organisms/authors/
- Madhaiyan M, Poonguzhali S, Lee JS, Senthilkumar M, Lee KC et al (2010) Mucilaginibacter gossypii sp. and Mucilaginibacter gossypiicola sp. nov., plant-growth-promoting bacteria isolated from cotton rhizosphere soils. Int J syst Evol Microbiol 60:2451–2357. https://www.microbiologyresearch.org/docserver/fulltext/ijsem/60/10/2451.pdf?expires=1568098026&id=id&accname=guest&checksum=618CFD2997ADBE0F19B6D9339FE8F45F
- Männistö MK, Tiirola M, McConnell J, Häggblom MM (2010) Mucilaginibacter frigoritolerans sp. nov., Mucilaginibacter lappiensis sp. nov. and Mucilaginibacter mallensis sp. nov., isolated from soil and lichen samples. Int J Syst Evol Microbiol 60:2849–2856. https://doi.org/10.1099/ijs.0.019364-0 CrossRefGoogle Scholar
- Pankratov TA, Tindall BJ, Liesack W, Dedysh SN (2007) Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog. Int J Syst Evol Microbiol 57:2349–2354. https://doi.org/10.1099/ijs.0.65100-0 CrossRefGoogle Scholar
- Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4(4):406–425Google Scholar
- Sasser M (2001) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101 DE: MIDI Inc, NewarkGoogle Scholar
- Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC205328/pdf/jbacter00026-0002.pdf