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Mucilaginibacter hurinus sp. nov., isolated from briquette warehouse soil

  • Lina Choi
  • Xinran Zhao
  • Yali Song
  • Minghan Wu
  • Gejiao Wang
  • Mingshun LiEmail author
Original Paper
  • 7 Downloads

Abstract

A novel bacterial strain, designated ZR32T, was isolated from briquette warehouse soil in Ulsan (Korea). The strain was aerobic, showing pink-colored colonies on R2A agar. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ZR32T was closely related to Mucilaginibacter soli R9-65T (97.0%), Mucilaginibacter gynuensis YC7003T (96.9%), and Mucilaginibacter lutimaris BR-3T (96.8%). The values of DNA–DNA relatedness related two highest strains M. soli R9-65T and M. gynuensis YC7003T were 31.2 ± 6.9% and 19.7 ± 0.3%, respectively. Its genome size was 3.9 Mb, comprising 3402 predicted genes. The DNA G+C content of strain ZR32T was 43.0 mol%. The major cellular fatty acids (> 5% of total) were summed feature 3 (C16:1ω6c and/or C16:1ω7c), C16:0, C16:1ω5c, iso-C15:0, iso-C17:0 3-OH, and C17:1ω9c. The major respiratory quinine was menaquinone-7 (MK-7). The major polar lipids were phosphatidylethanolamine, two unidentified phospholipids, one unidentified sphingolipid, and one unidentified polar lipid. Strain ZR32T showed distinctive characteristics such as the temperature and pH for growth ranges, being positive for β-glucosidase, salicin production, negative for N-acetyl-glucosamine assimilation, being resistant to carbenicillin and piperacillin to related species. On the basis of phenotypic, chemotaxonomic, and phylogenetic data, strain ZR32T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter hurinus sp. nov. is proposed. The type strain is ZR32T (= KCTC 62193 = CCTCC AB 2017285).

Keywords

Mucilaginibacter hurinus Polyphasic taxonomy 

Abbreviations

KCTC

Korean Collection for Type Cultures

NRRL

Agricultural Research Service Culture Collection

CCTCC

China Center for Type Culture Collection

KACC

Korean Agricultural Culture Collection

TEM

Transmission electron microscopy

MK-7

Menaquinone-7

MK-6

Menaquinone-6

NCBI

National Center for Biotechnology Information

LB

Luria–Bertani

NA

Nutrient agar

TSA

Trypticase soy agar

MH

Mueller–Hinton

MEGA

Molecular evolutionary genetics analysis

GDDCM

Guangdong Detection Center of Microbiology

Notes

Acknowledgements

This work was supported by the Development Program of China (National Development and Reform Commission; 2016YFD0800702). The authors are grateful to Prof. Dr. Bernhard Schink for the Latin construction of the species name. We acknowledge the Guangdong Detection Center of Microbiology (GDDCM) for fatty acid composition, polar lipids, and the DNA–DNA hybridization analyses. In addition, Genome was analyzed at Wuhan Frasergen Bioinformatics Co., Ltd.

Author contributions

LC: she has brought soil sample from Korea. She supported the procedures for the characterization for the strain ZR32T and the closest type strains. She conducted the PCR and sequencing of the 16S rRNA gene. She had constructed the phylogenetic trees, and Gram staining of the cells. She determined the cellular fatty acids. She had prepared the lyophilized powder of strain ZR32T and the closest related strains for analysis of polar lipids, isoprenoid quinone and DNA–DNA hybridization. She had conducted the experiments for bacterial phenotypic characteristics using the API strips. She had performed the analysis of the genome of ZR32T. She also made the tables and figure and wrote the manuscript. XZ: She helped Ms. Choi to collect colonies. In addition, she had conducted the experiments for bacterial phenotypic characteristics (growth temperature test, growth pH test, growth NaCl test, and motility test). YS: she supported the whole methods for the characterization for the strain ZR32T and the closest type strains. MW: he helped to correct grammar errors. In addition, he helped to revise the paper. GW: she supported the funding for the study. In addition, she advised about the way of experiments. ML: she proposed strain ZR32T as a new species. In addition, she advised about experimental skills.

Compliance with ethical standards

Conflict of interest

There is no conflict of interest.

Supplementary material

203_2019_1720_MOESM1_ESM.docx (749 kb)
Supplementary file1 (DOCX 748 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Lina Choi
    • 1
  • Xinran Zhao
    • 1
  • Yali Song
    • 1
  • Minghan Wu
    • 1
  • Gejiao Wang
    • 1
  • Mingshun Li
    • 1
    Email author
  1. 1.State Key Laboratory of Agricultural Microbiology, College of Life Science and TechnologyHuazhong Agricultural UniversityWuhanPeople’s Republic of China

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