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Effect of static magnetic field (200 mT) on biofilm formation in Pseudomonas aeruginosa

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Abstract

Several studies have investigated the effects of ionizing and non-ionizing radiations on microorganisms. However, the interaction between the magnetic field radiations and bacteria is less studied. The aim of our study was to study the effect of static magnetic field on the biofilm formation in Pseudomonas aeruginosa and its isogenic sod mutants. Our results revealed that the exposure to the static magnetic field (200 mT) increases significantly the swarming in the wild strain. The fliC gene expression did not show significant difference after 6 h exposure of the wild-type strain. The release of some compounds of the biofilm matrix such as rhamnolipids has been considerably enhanced after 6 h of exposure in the wild type. On the other hand, the pyocyanin and biofilm production was increased significantly in all strains compared to controls. Furthermore, our results revealed that the biofilm formation was confirmed by the pslA and ppyR gene expressions.

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Acknowledgements

The authors are grateful to Mr Iiyama Kazuhiro of the Fukuoka University of Agriculture for providing bacterial strains.

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Authors and Affiliations

  1. Biochemistry and Molecular Biology Unit, Faculty of Science of Bizerte, Carthage University, 7021, Jarzouna, Tunisia

    Hanini Raouia, Beghalem Hamida, Aliliche Khadidja & Landoulsi Ahmed

  2. Wastewater Treatment and Valorization Laboratory, Water Research and Technology Center CERTE, Technopole Borj Cedria, 8020, Soliman, Tunisia

    Chatti Abdelwaheb

Authors
  1. Hanini Raouia
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  2. Beghalem Hamida
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  3. Aliliche Khadidja
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  4. Landoulsi Ahmed
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  5. Chatti Abdelwaheb
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Correspondence to Hanini Raouia.

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Communicated by Jorge Membrillo-Hernández.

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Raouia, H., Hamida, B., Khadidja, A. et al. Effect of static magnetic field (200 mT) on biofilm formation in Pseudomonas aeruginosa. Arch Microbiol 202, 77–83 (2020). https://doi.org/10.1007/s00203-019-01719-8

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