Cupriavidus sp. strain Ni-2 resistant to high concentration of nickel and its genes responsible for the tolerance by genome comparison

  • Seul Lee
  • Anamika Khanal
  • A-Hyeon Cho
  • Hyeri Lee
  • Myung-Suk Kang
  • Tatsuya Unno
  • Hor-Gil Hur
  • Ji-Hoon LeeEmail author
Original Paper


The widespread use of metals influenced many researchers to examine the relationship between heavy metal toxicity and bacterial resistance. In this study, we have inoculated heavy metal-contaminated soil from Janghang region of South Korea in the nickel-containing media (20 mM Ni2+) for the enrichment. Among dozens of the colonies acquired from the several transfers and serial dilutions with the same concentrations of Ni, the strain Ni-2 was chosen for further studies. The isolates were identified for their phylogenetic affiliations using 16S rRNA gene analysis. The strain Ni-2 was close to Cupriavidus metallidurans and was found to be resistant to antibiotics of vancomycin, erythromycin, chloramphenicol, ampicillin, gentamicin, streptomycin, and kanamycin by disk diffusion method. Of the isolated strains, Ni-2 was sequenced for the whole genome, since the Ni-resistance seemed to be better than the other strains. From the genome sequence we have found that there was a total of 89 metal-resistance-related genes including 11 Ni-resistance genes, 41 heavy metal (As, Cd, Zn, Hg, Cu, and Co)-resistance genes, 22 cation-efflux genes, 4 metal pumping ATPase genes, and 11 metal transporter genes.


Heavy metal resistance Nickel Cupriavidus metallidurans Genome sequence 



This research was supported by National Institute of Biological Resources, South Korea, and in part by “Basic Science Research Program” through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A3B01012231).

Compliance with ethical standards

Conflict of interest

None declared.

Supplementary material

203_2019_1700_MOESM1_ESM.docx (746 kb)
Supplementary material 1 (DOCX 745 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Bioenvironmental ChemistryChonbuk National UniversityJeonju-siRepublic of Korea
  2. 2.National Institute of Biological ResourcesIncheonRepublic of Korea
  3. 3.Faculty of BiotechnologyJeju National UniversityJejuRepublic of Korea
  4. 4.School of Earth Sciences and Environmental EngineeringGwangju Institute of Science and TechnologyGwangjuRepublic of Korea

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