Intra-amoebic localization of Arcobacter butzleri as an endocytobiont of Acanthamoeba castellanii
Acanthamoeba castellanii is a free-living amoeba found mainly in humid environments and Arcobacter butzleri is an emerging zoonotic pathogen, both can establish in vitro endosymbiotic relationships in the absence of bacterial replication. We analyzed the localization of A. butzleri within A. castellanii establishing their association with endoplasmic reticulum vesicles and mitochondria. Through confocal microscopy, we observed that during the early stages of endosymbiosis, there is not colocalization between amoebic vacuoles containing A. butzleri and mitochondria or ER vesicles of A. castellanii. Considering that energy production of this bacterium occurs via metabolism of amino acids or the tricarboxylic acid cycle, these results contribute to explain the absence of bacterial replication, since A. butzleri would not have access to the nutrients found in endoplasmic reticulum vesicles and mitochondria. In addition, we observe that A. butzleri induces significantly the actin polymerization of A. castellanii during the early stages of endosymbiosis.
KeywordsAcanthamoeba Arcobacter Endosymbiosis Confocal microscopy
This work was supported by grants Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT) 1110202, Comisión Nacional de Investigación Científica y Tecnológica (CONICYT) AT 24121322 from Chile and Fondo de Equipamiento FEQUIP2018-PL-06 Vicerrectoría de Investigación y Posgrado Universidad Católica de Temuco. The authors would like to thank Professor Ph.D. Guillermo Pérez-Pérez (New York University Langone Medical Center) for their scientific advices and critical review.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Fernández H, Villanueva MP, Medina G (2012) Endosymbiosis of Arcobacter butzleri in Acanthamoeba castellanii. Rev Argent Microbiol 44:133Google Scholar
- Gilson PR, Yu X-C, Hereld D et al (2003) Two Dictyostelium orthologs of the prokaryotic cell division protein FtsZ localize to mitochondria and are required for the maintenance of normal mitochondrial morphology. Eukaryot Cell 2:1315–1326. https://doi.org/10.1128/EC.2.6.1315-1326.2003 CrossRefGoogle Scholar
- Oliver JD (2005) The viable but nonculturable state in bacteria. J Microbiol 43:93–100Google Scholar
- Pizarro-Cerdá J, Méresse S, Parton RG et al (1998) Brucella abortus transits through the autophagic pathway and replicates in the endoplasmic reticulum of nonprofessional phagocytes. Infect Immun 66:5711–5724Google Scholar