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Effect of Fe on inorganic polyphosphate level in autotrophic and heterotrophic cells of Rhodospirillum rubrum

  • Tatiana KulakovskayaEmail author
  • Anton Zvonarev
  • Kestutis Laurinavichius
  • Galina Khokhlova
  • Mikhail Vainshtein
Short Communication
  • 57 Downloads

Abstract

Inorganic polyphosphate is involved in metal homeostasis in microorganisms. The aim of the study was to reveal differences in polyphosphate metabolism of Rhodospirillum rubrum under autotrophic and heterotrophic cultivation in the presence of Fe (2.3 mg Fe3+ L−1) and without Fe (traces). Heterotrophic conditions without Fe resulted in cell lysis and low biomass yield. High polyphosphate content and low exopolyphosphatase activity were observed in the cells cultivated autotrophically in the presence of Fe. The cells grown heterotrophically in the presence of Fe contained more phosphate and low-molecular polyphosphate; on the contrary, the content of the high molecular polyphosphate decreased in parallel with the increase in exopolyphosphatase activity. The possible involvement of Pi and polyphosphate to the formation of Fe-containing inclusions is discussed.

Keywords

Rhodospirillum rubrum Polyphosphate Polyphosphatase Fe Autotrophic growth Heterotrophic growth 

Notes

Supplementary material

203_2019_1697_MOESM1_ESM.pdf (537 kb)
Supplementary material 1 (PDF 536 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.G.K. Skryabin Institute of Biochemistry and Physiology of MicroorganismsFRC Pushchino Scientific Center for Biological Research of the Russian Academy of SciencesPushchinoRussia

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