Archives of Microbiology

, Volume 201, Issue 9, pp 1259–1275 | Cite as

Proteomics-based discrimination of differentially expressed proteins in antibiotic-sensitive and antibiotic-resistant Salmonella Typhimurium, Klebsiella pneumoniae, and Staphylococcus aureus

  • Md Jalal Uddin
  • Choong Je Ma
  • Jin-Chul Kim
  • Juhee AhnEmail author
Original Paper


This study was designed to compare the differentially expressed proteins between antibiotic-sensitive and antibiotic-resistant Salmonella Typhimurium, Klebsiella pneumonia, and Staphylococcus aureus. The susceptibilities of wild-type (WT), ciprofloxacin (CIP) and/or oxacillin (OXA)-induced, and clinically isolated resistant (CCARM) S. Typhimurium (STWT, STCIP, and STCCARM), K. pneumoniae (KPWT, KPCIP, and KPCCARM), and S. aureus (SAWT, SACIP, SAOXA, and SACCARM) to antibiotics were determined using broth microdilution assay. STCIP was highly resistant to piperacillin (MIC > 512 μg/ml), KPCIP was resistant to chloramphenicol (128 μg/ml) and norfloxacin (16 μg/ml), SACIP was resistant to fluoroquinolones (32 μg/ml), and SAOXA was resistant to ceftriaxone (32 μg/ml). The protein profiles of antibiotic-sensitive and antibiotic-resistant strains were determined using 2-DE analysis followed by LC–MS/MS. The commonly expressed proteins of STWT–STCIP, STWT–STCCARM, KPWT–KPCIP, KPWT–KPCCARM, SAWT–SACIP, SAWT–SAOXA, and SAWT–SACCARM were 763, 677, 677, 469, 261, 259, and 226, respectively. The unique protein spots were observed 57 (6.5%), 80 (11.5%), and 68 (13.9%), respectively, for STCCARM, KPCCARM, and SACCARM. The highly up-regulated protein, PrsA (10-fold), was observed in STCIP resistant to ciprofloxacin (128-fold), levofloxacin (32-fold), norfloxacin (64-fold), and piperacillin (> 16-fold). The up-regulated proteins (YadC, FimA, and RplB) in KPCIP resistant to chloramphenicol (> 32-fold), ciprofloxacin (32-fold), levofloxacin (6-fold), norfloxacin (128-fold), and sparfloxacin (64-fold). AcrB and RpoB were up-regulated in SACCARM resistant to multiple antibiotics. The differentially expressed proteins were related to the antibiotic resistance of STWT, STCIP, STCCARM, KPWT, KPCIP, KPCCARM, SAWT, SACIP, SAOXA, and SACCARM. The resistance-associated proteins could be useful biomarkers for detecting antibiotic-resistant pathogens.


Antibiotic resistance Proteome Salmonella Klebsiella Staphylococcus 



This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (Grant number: HI15C-1798-000016).

Supplementary material

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Supplementary material 1 (DOCX 24 kb)
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Supplementary material 2 (DOCX 25 kb)
203_2019_1693_MOESM3_ESM.docx (27 kb)
Supplementary material 3 (DOCX 27 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Medical Biomaterials Engineering and Institute of Bioscience and BiotechnologyKangwon National UniversityChuncheonRepublic of Korea

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