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Archives of Microbiology

, Volume 201, Issue 9, pp 1285–1293 | Cite as

An endophytic Kocuria palustris strain harboring multiple arsenate reductase genes

  • Tania Zacaria Vital
  • Brenda Román-Ponce
  • Flor N. Rivera Orduña
  • Paulina Estrada de los Santos
  • M. Soledad Vásquez-Murrieta
  • Yue Deng
  • Hong Li Yuan
  • En Tao WangEmail author
Original Paper

Abstract

Aiming at revealing the arsenic (As) resistance of the endophytic Kocuria strains isolated from roots and stems of Sphaeralcea angustifolia grown at mine tailing, four strains belonging to different clades of Kocuria based upon the phylogeny of 16S rRNA genes were screened for minimum inhibitory concentration (MIC). Only the strain NE1RL3 was defined as an As-resistant bacterium with MICs of 14.4/0.0125 mM and 300/20.0 mM for As3+ and As5+, respectively, in LB/mineral media. This strain was identified as K. palustris based upon analyses of cellular chemical compositions (cellular fatty acids, isoprenoides, quinones, and sugars), patterns of carbon source, average nucleotide identity of genome and digital DNA–DNA relatedness. Six genes coding to enzymes or proteins for arsenate reduction and arsenite-bumping were detected in the genome, demonstrating that this strain is resistant to As possibly by reducing As5+ to As3+, and then bumping As3+ out of the cell. However, this estimation was not confirmed since no arsenate reduction was detected in a subsequent assay. This study reported for the first time the presence of phylogenetically distinct arsenate reductase genes in a Kocuria strain and evidenced the possible horizontal transfer of these genes among the endophytic bacteria.

Keywords

Endophyte Genome Arsenate reductase Polyphasic identification 

Notes

Acknowledgements

This study was financially supported by the project SIP20171259 authorized by Instituto Politecnico Nacional (IPN), Mexico. TZV received scholarships of CONACyT of Mexico and PIFI (IPN) for her study and staying 2 months in the laboratory of Dr. Yuan. FNRO, PES, MSVM and ETW thank for the scholarships of SNI authorized by CONACyT, EDI and COFAA PIFI (IPN) for her study and staying 2 months in the laboratory of Dr. Yuan. FNRO, PES, MSVM and ETW thank for the scholarships of SNI authorized by CONACyT, EDI and COFAA authorized by IPN.

Supplementary material

203_2019_1692_MOESM1_ESM.docx (698 kb)
Supplementary material 1 (DOCX 697 kb)
203_2019_1692_MOESM2_ESM.docx (16 kb)
Supplementary material 2 (DOCX 16 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Tania Zacaria Vital
    • 1
  • Brenda Román-Ponce
    • 1
    • 3
  • Flor N. Rivera Orduña
    • 1
  • Paulina Estrada de los Santos
    • 1
  • M. Soledad Vásquez-Murrieta
    • 1
  • Yue Deng
    • 2
  • Hong Li Yuan
    • 2
  • En Tao Wang
    • 1
    Email author
  1. 1.Departamento de Microbiología, Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalMexico CityMexico
  2. 2.State Key Laboratory of Agrobiotechnology, College of Biological Sciences and Rhizobium Research CenterChina Agricultural UniversityBeijingChina
  3. 3.Departamento de Microbiología y Genética, Edificio Departamental, Lab. 214, Campus Miguel de UnamunoUniversidad de SalamancaSalamancaSpain

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