Archives of Microbiology

, Volume 201, Issue 9, pp 1195–1205 | Cite as

Purification, characterization and antibacterial spectrum of a compound produced by Bacillus cereus MTCC 10072

  • Narender kumar
  • Namita SinghEmail author
  • Rajneesh Jaryal
  • Chetna Bhandari
  • Jyoti Singh
  • Pallavi Thakur
  • Anil Duhan
Original Paper


Awareness of the consumer has increased the demand of safe and chemical-free foods, and consequently it has increased the demand of antibacterial bioactive compounds. In the present study, antibacterial compound produced by a local bacterial isolate NSD MTCC 10072, showing antagonistic activity against six human pathogens, was isolated, partially purified and characterized. Maximum production of antibacterial compound was observed between 51 and 60 h after seeding. The antibacterial activity of the compound was found to be thermostable up to 80 °C for 60 min and its efficacy was very good between pH 4 and 12. Minimum inhibitory concentration (25.84 µg/µl) of the antibacterial compound was observed against Streptococcus aureus NICM 2901. GC–MS analysis of the bacterium secreted chemical compound (C11H18N2O2) was used to identify the antimicrobial compound as Pyrrolo(1,2-a) pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl). In Silico studies showed that the antimicrobial compound is non-toxic, non-irritating and followed Lipinski-type properties which suggested that the compound could be used as potential drug against different human pathogens.


Bacillus Optimisation Cytotoxicity Antibacterial Bio-control 



Authors are grateful to the University Grant Commission (UGC SAP), Department of Science and Technology (A-2/1754-57 dated 01/06/2016) and Haryana State Council of Science and Technology (HSCST 1803 dated 20/04/2016; HSCST 72/01 dated 01/01/2017) for providing the financial assistance under the MRP scheme, infrastructure facilities and fellowship, respectively.

Compliance with ethical standards

Conflict of interest

There is no conflict of interest between the authors.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Narender kumar
    • 1
  • Namita Singh
    • 1
    Email author
  • Rajneesh Jaryal
    • 1
  • Chetna Bhandari
    • 1
  • Jyoti Singh
    • 1
  • Pallavi Thakur
    • 1
    • 2
  • Anil Duhan
    • 3
  1. 1.Microbial Biotechnology laboratory, Department of Bio and Nano TechnologyGuru Jambheshwar University of Science and TechnologyHisarIndia
  2. 2.Division of CBRN DefenceInstitute of Nuclear Medicine and Allied SciencesDelhiIndia
  3. 3.Department of AgronomyCCS Haryana Agricultural UniversityHisarIndia

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