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Molecular identification of clinical and environmental avian Aspergillus isolates

  • Raquel Sabino
  • Julia Burco
  • Joana Valente
  • Cristina Veríssimo
  • Karl V. Clemons
  • David A. Stevens
  • Lisa A. Tell
Short Communication
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Abstract

Aspergillosis causes high morbidity and mortality in avian species. The main goal of this study was to use molecular techniques to identify Aspergillus species collected from different avian species with aspergillosis. A subsample of those isolates was also screened for resistance to itraconazole. Over a 2-year period, clinical samples were recovered from 44 birds with clinical signs of the disease, clinical pathology results suspicious of aspergillosis, or from birds that died from Aspergillus spp. infection. Environmental sampling was also performed in seabird rehabilitation centers and natural seabird environments. Seventy-seven isolates (43 clinical and 34 environmental) were identified as Aspergillus fumigatus sensu stricto. No cryptic species from the Fumigati section were detected. Two environmental isolates were identified as Aspergillus nidulans var. dentatus and Aspergillus spinulosporus. None of the Aspergillus isolates tested were resistant to itraconazole. Our study emphasizes the dominant association of Aspergillus fumigatus sensu stricto in avian mycoses and shows the lack of itraconazole resistance in the studied isolates.

Keywords

Aspergillus Cryptic species Birds Aspergillosis Drug susceptibility 

Notes

Acknowledgements

The authors would like to thank Spencer Jang and Anita Wong for their assistance with gathering the clinical isolates.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.URSZ-Infectious Diseases DepartmentNacional Institute of Health Dr. Ricardo JorgeLisbonPortugal
  2. 2.Oregon Department of Fish and WildlifeCorvallisUSA
  3. 3.Departamento de Ciências e Tecnologia da BiomassaFaculdade de Ciências e Tecnologia da Universidade Nova de LisboaLisbonPortugal
  4. 4.Division of Infectious Diseases and Geographic Medicine, Department of MedicineStanford UniversityStanfordUSA
  5. 5.California Institute for Medical ResearchSan JoseUSA
  6. 6.Department of Medicine and Epidemiology, School of Veterinary MedicineUniversity of CaliforniaDavisUSA

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