Archives of Microbiology

, Volume 201, Issue 2, pp 245–251 | Cite as

Pseudomonas humi sp. nov., isolated from leaf soil

  • Hironaga Akita
  • Zen-ichiro KimuraEmail author
  • Tamotsu Hoshino
Original Paper


An aerobic, Gram-negative, non-sporulating, motile, rod-shaped and lignin-degrading bacterial strain, Pseudomonas sp. CCA1, was isolated from leaf soil collected in Japan. This strain grew at 20–45 °C (optimum 20 °C), at pH 5.0–10.0 (optimum pH 5.0), and in the presence of 2% NaCl. Its major cellular fatty acids were C16:0 and summed feature 8 (C18:1ω6c and/or C18:1ω7c). The predominant quinone system was ubiquinone-9. Comparative 16S rRNA gene sequence analysis showed that Pseudomonas sp. CCA1 was related most closely to P. citronellolis NBRC 103043T (98.9%), but multilocus sequence analysis based on fragments of the atpD, gyrA, gyrB and rpoB gene sequences showed strain CCA1 to branch separately from its most closely related Pseudomonas type strains. DNA–DNA hybridization values between Pseudomonas sp. CCA1 and type strains of closely related Pseudomonas species were less than 53%. Based on its phenotypic, chemotaxonomic and phylogenetic features, we propose that Pseudomonas sp. CCA1 represents a novel species within the genus Pseudomonas, for which the name Pseudomonas humi sp. nov. is proposed. The type strain is CCA1 (= HUT 8136T = TBRC 8616T).


Pseudomonas humi sp. nov. Multilocus sequence analysis DNA–DNA hybridization Lignin degradation Leaf soil 



This work was supported in part by the Science and Technology Research Partnership for Sustainable Development (SATREPS), under the Japan Science and Technology Agency (JST) and Japan International Cooperation Agency (JICA).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

203_2018_1588_MOESM1_ESM.pdf (159 kb)
Supplementary material 1 (PDF 158 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Research Institute for Sustainable ChemistryNational Institute of Advanced Industrial Science and Technology (AIST)Higashi-HiroshimaJapan
  2. 2.Department of Civil and Environmental Engineering, National Institute of TechnologyKure CollegeKureJapan
  3. 3.Bioproduction Research InstituteNational Institute of Advanced Industrial Science and Technology (AIST)SapporoJapan

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