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Marinactinospora rubrisoli sp. nov., isolated from red soil

  • Bing Huang
  • Kun Cheng
  • Limin Zhang
  • Ying Huang
  • Changting Liu
Original Paper
  • 5 Downloads

Abstract

A novel Marinactinospora strain JX35-4T was isolated from red soil which was collected from Wushan, northern Jiangxi Province, China. Analysis of the 16S rRNA gene sequences showed that strain JX35-4T belongs to the genus Marinactinospora and formed a distinct phylogenetic clade with Marinactinospora thermotolerans SCSIO 00652T and Marinactinospora endophytica YIM 690053T with sequence similarity of 96.97% and 96.42%, respectively. The strain was Gram-positive and formed branched substrate hyphae with no fragmentation, and abundant aerial hyphae that differentiated into long spore chains, and short rod-shaped spores. Growth occurred at 20–45 °C, pH 7.0–12.0 and in the presence of 0–7.5% (w/v) NaCl. The genomic DNA G + C content was determined to be 68.3 mol%. The cell wall of strain JX35-4T contained meso-diaminopimelic acid and xylose. Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol mannosides and one unidentified phospholipid. The major fatty acids of strain JX35-4T consisted of anteiso-C17:0 and iso-C16:0. Major menaquinones were MK-9(H10), MK-12 and MK-10(H2). Based on the polyphasic data, strain JX35-4T (= CGMCC 4.7382T = DSM 104977T) is concluded to represent a novel species of the genus Marinactinospora, for which the name Marinactinospora rubrisoli sp. nov. is proposed.

Keywords

Marinactinospora rubrisoli sp. nov. Red soil Polyphasic taxonomy 16S rRNA gene 

Notes

Acknowledgements

The authors are grateful to Prof. Jisheng Ruan (Institute of Microbiology, Chinese Academy of Sciences, IMCAS) for suggesting the Latin name of the species, and to Prof. Chunli Li (IMCAS) for his help in SEM analyses. This work was supported by the National Program on Key Basic Research Project (973 Program, no. 2014CB744400), Major Project of Military Medicine (no. BWS17C030) and the General Financial Grant from the China Postdoctoral Science Foundation (no. 2016M602971).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

203_2018_1583_MOESM1_ESM.docx (1.7 mb)
Supplementary material 1 (DOCX 1747 KB)

References

  1. Akhwale JK, Goker M, Rohde M, Schumann P, Boga HI, Klenk HP (2016) Nocardiopsis mwathae sp. nov., isolated from the haloalkaline Lake Elmenteita in the African Rift Valley. Antonie Van Leeuwenhoek 109:421–430.  https://doi.org/10.1007/s10482-016-0647-z CrossRefPubMedGoogle Scholar
  2. Buck JD (1982) Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993PubMedPubMedCentralGoogle Scholar
  3. Chen Q, Ji C, Song Y, Huang H, Ma J, Tian X, Ju J (2013) Discovery of McbB, an enzyme catalyzing the beta-carboline skeleton construction in the marinacarboline biosynthetic pathway. Angew Chem Int Ed Engl 52:9980–9984.  https://doi.org/10.1002/anie.201303449 CrossRefPubMedGoogle Scholar
  4. Chen E, Chen Q, Chen S, Xu B, Ju J, Wang H (2017) Mathermycin, a lantibiotic from the marine actinomycete Marinactinospora thermotolerans SCSIO 00652. Appl Environ Microbiol.  https://doi.org/10.1128/aem.00926-17 CrossRefPubMedPubMedCentralGoogle Scholar
  5. Collins MD, Jones D (1980) Lipids in the classification and identification of Coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. J Appl Microbiol 48:459–470.  https://doi.org/10.1111/j.1365-2672.1980.tb01036.x CrossRefGoogle Scholar
  6. Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230.  https://doi.org/10.1099/00221287-100-2-221 CrossRefPubMedGoogle Scholar
  7. Cui XL, Mao PH, Zeng M, Li WJ, Zhang LP, Xu LH, Jiang CL (2001) Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51:357–363.  https://doi.org/10.1099/00207713-51-2-357 CrossRefPubMedGoogle Scholar
  8. Euzeby JP (1997) List of bacterial names with standing in nomenclature: a folder available on the internet. Int J Syst Bacteriol 47:590–592.  https://doi.org/10.1099/00207713-47-2-590 CrossRefPubMedGoogle Scholar
  9. Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376CrossRefGoogle Scholar
  10. Felsenstein J (1985) Confidence limits on phylogeny: an approach using. the bootstrap Evolution 39:783–791.  https://doi.org/10.1111/j.1558-5646.1985.tb00420.x CrossRefPubMedGoogle Scholar
  11. Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416CrossRefGoogle Scholar
  12. Gonzalez C, Gutierrez C, Ramirez C (1978) Halobacterium vallismortis sp. nov. an amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24:710–715CrossRefGoogle Scholar
  13. Guo X, Liu N, Li XM, Ding Y, Shang F, Gao YS, Ruan JS, Huang Y (2015) Red soils harbor diverse culturable actinomycetes that are promising sources of novel secondary metabolites. Appl Environ Microbiol 81:3086–3103.  https://doi.org/10.1128/aem.03859-14 CrossRefPubMedPubMedCentralGoogle Scholar
  14. Huang H, Yao Y, Yang T, Ma J, Tian X, Li Y, Huang C, Chen X, Li W, Zhang S, Zhang C, Ju J (2011) Antimalarial beta-carboline and indolactam alkaloids from Marinactinospora thermotolerans, a deep sea isolate. J Nat Prod 74:2122–2127.  https://doi.org/10.1021/np200399t CrossRefPubMedGoogle Scholar
  15. Kelly KL (1964) Inter-society color council-national bureau of standards color name charts illustrated with centroid colors. US Government Printing Office, Washington, DCGoogle Scholar
  16. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120CrossRefGoogle Scholar
  17. Lee SB, Strand SE, Stensel HD (2000) Sustained degradation of trichloroethylene in a suspended growth gas treatment reactor by an actinomycetes enrichment. Environ Sci Technol 34:3261–3268.  https://doi.org/10.1021/es9907515 CrossRefGoogle Scholar
  18. Li WJ, Kroppenstedt RM, Wang D, Tang SK, Lee JC, Park DJ, Kim CJ, Xu LH, Jiang CL (2006) Five novel species of the genus Nocardiopsis isolated from hypersaline soils and emended description of Nocardiopsis salina Li et al. 2004. Int J Syst Evol Microbiol 58:1089–1096.  https://doi.org/10.1099/ijs.0.64033-0 CrossRefGoogle Scholar
  19. Liu MJ, Khieu TN, Gao R, Hozzein WN, Wang HF, Yang W, Nimaichand S, Xiong Z, Duan YQ, Li WJ (2015) Marinactinospora endophytica sp. nov., isolated from a medicinal plant. Antonie Van Leeuwenhoek 107:1577–1582.  https://doi.org/10.1007/s10482-015-0451-1 CrossRefPubMedGoogle Scholar
  20. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167.  https://doi.org/10.1099/00207713-39-2-159 CrossRefGoogle Scholar
  21. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241.  https://doi.org/10.1016/0167-7012(84)90018-6 CrossRefGoogle Scholar
  22. Muangham S, Suksaard P, Mingma R, Matsumoto A, Takahashi Y, Duangmal K (2016) Nocardiopsis sediminis sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 66:3835–3840.  https://doi.org/10.1099/ijsem.0.001273 CrossRefPubMedGoogle Scholar
  23. Oren A, Garrity GM (2015) List of new names and new combinations previously effectively, but not validly, published. Int J Syst Evol Microbiol 65:2777–2783.  https://doi.org/10.1099/ijsem.0.000464 CrossRefGoogle Scholar
  24. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425.  https://doi.org/10.1093/oxfordjournals.molbev.a040454 CrossRefPubMedGoogle Scholar
  25. Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340.  https://doi.org/10.1099/00207713-16-3-313 CrossRefGoogle Scholar
  26. Stackebrandt E, Ebers J (2006) Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155Google Scholar
  27. Staneck JL, Roberts GD (1974) Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231PubMedPubMedCentralGoogle Scholar
  28. Tamaoka J, Katayama-Fujimura Y, Kuraishi H (1983) Analysis of bacterial menaqui-none mixtures by high performance liquid chromatography. J Appl Bacteriol 54:31–36CrossRefGoogle Scholar
  29. Tamura T, Hatano K (2001) Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and Actinoplanes aurantiacus’ to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov. Int J Syst Evol Microbiol 51:2119–2125.  https://doi.org/10.1099/00207713-51-6-2119 CrossRefPubMedGoogle Scholar
  30. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739.  https://doi.org/10.1093/molbev/msr121 CrossRefPubMedPubMedCentralGoogle Scholar
  31. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882.  https://doi.org/10.1093/nar/25.24.4876 CrossRefPubMedPubMedCentralGoogle Scholar
  32. Tian XP, Tang SK, Dong JD, Zhang YQ, Xu LH, Zhang S, Li WJ (2009) Marinactinospora thermotolerans gen. nov., sp. nov., a marine actinomycete isolated from a sediment in the northern South China Sea. Int J Syst Evol Microbiol 59:948–952.  https://doi.org/10.1099/ijs.0.005231-0 CrossRefPubMedGoogle Scholar
  33. Waksman SA (1961) The actinomycetes. vol. II. Classification, identification and descriptions of genera and species. Williams & Wilkins, BaltimoreGoogle Scholar
  34. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ, Chen HH, Xu LH, Jiang CL (2005) Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153.  https://doi.org/10.1099/ijs.0.63407-0 CrossRefPubMedGoogle Scholar
  35. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J (2017) Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. IntJ Syst Evol Microbiol 67:1613–1617.  https://doi.org/10.1099/ijsem.0.001755 CrossRefGoogle Scholar
  36. Zhu QH, Ma JY, Luo MH, Wang B, Huang HB, Tian XP, Li WJ, Zhang S, Zhang CS, Ju JH (2012) Discovery and engineered overproduction of antimicrobial nucleoside antibiotic A201A from the deep-sea marine actinomycete Marinactinospora thermotolerans SCSIO 00652. Antimicrob Agents Chemother 56:110–114.  https://doi.org/10.1128/aac.05278-11 CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Nanlou Respiratory Diseases DepartmentChinese PLA General Hospital/Chinese PLA Postgraduate Medical SchoolBeijingChina
  2. 2.State Key Laboratory of Microbial Resources, Institute of MicrobiologyChinese Academy of SciencesBeijingChina

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