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Archives of Microbiology

, Volume 201, Issue 1, pp 27–33 | Cite as

Cystobasidium keelungensis sp. nov., a novel mycosporine producing carotenogenic yeast isolated from the sea surface microlayer in Taiwan

  • Chin-Feng Chang
  • Ching-Fu Lee
  • Shiu-Mei Liu
Original Paper

Abstract

Cystobasidium keelungensis SN2T (CBS 6949 = BCRC 920080), a new anamorphic basidiomycetous yeast species, is described in this paper. The strains belonging to this species were isolated from the sea surface microlayer and underlying water in Taiwan. These strains were identified by examining nucleotide sequences in the species-specific D1/D2 domains of the large subunit (LSU) ribosomal RNA (rRNA) and by evaluating their physiological characteristics. Phylogenetic analyses of D1/D2 sequences revealed that C. keelungensis was most closely related to the species C. slooffiae (LSU rRNA gene sequence divergence of 1.5%), and it belonged to the Erythrobasidium clade. No sexual reproduction was observed. This species differed from related species in carbon and nitrogen assimilation patterns and growth at 35 °C. Screening C. keelungensis for the presence of UV-absorbing compounds showed that mycosporine–glutaminol–glucoside and mycosporine–glutamicol–glucoside (maximum absorption: 310 nm) were the major UV-absorbing compounds, which differ from the compounds present in some freshwater yeast strains reported in previous studies. After UV induction, SN2 had a higher level of mycosporine production than other carotenogenic yeasts in this study.

Keywords

New species Cystobasidium Mycosporine Sea surface microlayer 

Notes

Acknowledgements

This work was supported by Grants-in-Aid from the Ministry of Science and Technology, Executive Yuan, Taiwan (MOST 105-2311-B-157-001).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biological Science and TechnologyChina University of Science and TechnologyTaipeiTaiwan
  2. 2.Department of Applied ScienceNational Tsing Hua UniversityHsinchuTaiwan
  3. 3.Department of Biomedical Engineering and Environmental SciencesNational Tsing Hua UniversityHsinchuTaiwan
  4. 4.Institute of Marine BiologyNational Taiwan Ocean UniversitykeelungTaiwan

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