Archives of Microbiology

, Volume 201, Issue 1, pp 45–50 | Cite as

Winogradskyella profunda sp. nov. isolated from the Chukchi Sea bottom sediments

  • Lyudmila A. RomanenkoEmail author
  • Valeriya V. Kurilenko
  • Konstantin V. Guzev
  • Vassilii I. Svetashev
  • Valery V. Mikhailov
Original Paper


An aerobic, Gram-negative, yellow-pigmented non-motile rod-shaped bacterium, designated Ch38T, was isolated from a sediment sample collected from the Chukchi Sea in the Arctic Ocean. Comparative 16S rRNA gene sequence analysis positioned strain Ch38T into the genus Winogradskyella as a distinct line adjacent to Winogradskyella multivorans KCTC 23891T, sharing the highest similarities of 97.5%, 97.2%, and 97.1% with Winogradskyella eximia KCTC 12219T, Winogradskyella damuponensis KCTC 23552T, and Winogradskyella multivorans KCTC 23891T, respectively. Strain Ch38T grew at 5–36 °C and in the presence of 1–6% (w/v) NaCl. It contained MK-6 as the predominant menaquinone and iso-C16:0 3-OH, anteiso-C15:0 followed by iso-C15:0 and iso-C16:1 as the major fatty acids. Polar lipids consisted of phosphatidylethanolamine, three unknown aminolipids, an unknown lipid and an unknown phospholipid. The DNA C + C content was 31.7 mol%. Based on the phylogenetic analysis and distinctive phenotypic characteristics, strain Ch38T is concluded to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella profunda sp. nov. is proposed. The type strain of the species is strain Ch38T (= KMM 9725T = KACC 19710T).


Winogradskyella profunda sp. nov. Marine Bottom sediments 


Compliance with ethical standards

Conflict of interest

The authors have declared no conflict of interests.

Supplementary material

203_2018_1567_MOESM1_ESM.doc (6 mb)
Supplementary material 1 (DOC 6166 KB)


  1. Begum Z, Srinivas TNR, Manasa P, Sajlaja B, Sunil B, Prasad S, Shivaji S (2013) Winogradskyella psychrotolerans sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from Arctic sediment. Int J Syst Evol Microbiol 63:1646–1652CrossRefGoogle Scholar
  2. Bowman JP (2000) Description of Cellulophaga algicola sp. nov., isolated from the surface of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868CrossRefGoogle Scholar
  3. Bruns A, Rohde M, Berthe-Corti L (2001) Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006CrossRefGoogle Scholar
  4. Collins MD, Shah HN (1984) Fatty acid, menaquinone and polar lipid composition of Rothia dentosacariosa. Arch Microbiol 137:247–249CrossRefGoogle Scholar
  5. Edwards U, Rogall T, Blöcker H, Emde M, Böttger EC (1989) Isolation and direct complete nucleotide determination of entire genes: characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17:7843–7853CrossRefGoogle Scholar
  6. Fautz E, Reichenbach H (1980) A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8:87–91CrossRefGoogle Scholar
  7. Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509PubMedPubMedCentralGoogle Scholar
  8. Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) (1994) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DCGoogle Scholar
  9. Ivanova EP, Christen R, Gorshkova NM, Zhukova NV, Kurilenko VV, Crawford RJ, Mikhailov VV (2010) Winogradskyella exilis sp. nov., isolated from the starfish Stellaster equestris, and emended description of the genus Winogradskyella. Int J Syst Evol Microbiol 60:1577–1580CrossRefGoogle Scholar
  10. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721CrossRefGoogle Scholar
  11. Kim M, Oh HS, Park SC, Chun J (2014) Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 64:346–351CrossRefGoogle Scholar
  12. Lee DH, Cho SJ, Kim SM, Lee SB (2013) Winogradskyella damuponensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 63:321–326CrossRefGoogle Scholar
  13. Leifson E (1963) Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184PubMedPubMedCentralGoogle Scholar
  14. Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118CrossRefGoogle Scholar
  15. Mitchell K, Fallon RJ (1990) The determination of ubiquinone profiles by reversed-phase high performance thin-layer chromatography as an aid to the speciation of. Legionellaceae J Gen Microbiol 136:2035–2041CrossRefGoogle Scholar
  16. Nedashkovskaya OI, Kim SB, Han SK, Snauwaert C, Vancanneyt M, Swings J, Kim KO, Lysenko AM, Rohde M, Frolova GM, Mikhailov VV, Bae KS (2005) Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae. Int J Syst Evol Microbiol 55:49–55CrossRefGoogle Scholar
  17. Nedashkovskaya OI, Kukhlevskiy AD, Zhukova NV (2012) Winogradskyella ulvae sp. nov., an epiphyte of a Pacific seaweed, and emended descriptions of the genus Winogradskyella and Winogradskyella thalassocola, Winogradskyella echinorum, Winogradskyella exilis and Winogradskyella eximia. Int J Syst Evol Microbiol 62:1450–1456CrossRefGoogle Scholar
  18. Owen J, Hill LR, Lapage SP (1969) Determination of DNA base composition from melting profiles in dilute buffers. Biopolymers 7:503–516CrossRefGoogle Scholar
  19. Pearson W, Lipman DJ (1988) Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85:2444–2448CrossRefGoogle Scholar
  20. Romanenko LA, Uchino M, Frolova GM, Mikhailov VV (2007) Marixanthomonas ophiurae gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from a deep-sea brittle star. Int J Syst Evol Microbiol 57:457–462CrossRefGoogle Scholar
  21. Romanenko LA, Tanaka N, Svetashev VI (2013) Devosia submarina sp. nov., isolated from deep sea surface sediments. Int J Syst Evol Microbiol 63:3079–3085CrossRefGoogle Scholar
  22. Sasser M (1990) Microbial identification by gas chromatographic analysis of fatty acid methyl esters (GC-FAME).Technical Note 101. MIDI, NewarkGoogle Scholar
  23. Stackebrandt E, Ebers J (2006) Taxonomic parameters revisited: tarnished gold standards. Microb Today 45:153–155Google Scholar
  24. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefGoogle Scholar
  25. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Trüper HG (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464CrossRefGoogle Scholar
  26. Yoon JH, Lee SY (2012) Winogradskyella multivorans sp. nov., a polysaccharide-degrading bacterium isolated from seawater of an oyster farm. Antonie Van Leeuwenhoek 102:231–238CrossRefGoogle Scholar
  27. Yoon BJ, Byun HD, Kim JY, Lee DH, Kang HY, Oh DC (2011) Winogradskyella lutea sp. nov., isolated from seawater, and emended description of the genus Winogradskyella. Int J Syst Evol Microbiol 61:1539–1543CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Lyudmila A. Romanenko
    • 1
    Email author
  • Valeriya V. Kurilenko
    • 1
  • Konstantin V. Guzev
    • 1
  • Vassilii I. Svetashev
    • 2
  • Valery V. Mikhailov
    • 1
  1. 1.G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern BranchRussian Academy of SciencesVladivostokRussia
  2. 2.Institute of Marine Biology, Far Eastern BranchRussian Academy of SciencesVladivostokRussia

Personalised recommendations