Genome-wide analysis of differentially expressed lncRNA in sporadic parathyroid tumors
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Diagnosis of parathyroid carcinoma on histological examination is challenging. Thousands of differentially expressed lncRNAs were identified on the microarray data between parathyroid cancer and adenoma samples. Four lncRNAs were significantly dysregulated in further validation. The “lncRNA score” calculated from these lncRNAs differentiated parathyroid carcinomas from adenomas. LncRNAs serve as biomarkers for parathyroid cancer diagnosis.
Diagnosis of parathyroid carcinoma (PC) on histological examination is challenging. LncRNA profile study was conducted to find diagnostic biomarkers for PC.
LncRNA arrays containing 91,007 lncRNAs as well as 29,857 mRNAs were used to assess parathyroid specimen (5 carcinomas and 6 adenomas). Bioinformatics analyses were also conducted to compare the microarray results between parathyroid carcinomas and adenomas (PAs). Differentially expressed lncRNAs of 11 PCs and 31 PAs were validated by real-time quantitative PCR.
On the microarray data between PC and PA samples (fold change ≥ 2, P < 0.05), 1809 differentially expressed lncRNAs and 1349 mRNAs also were identified. All carcinomas were clustered in the same group by clustering analysis using dysregulated lncRNAs or mRNAs. Four lncRNAs (LINC00959, lnc-FLT3-2:2, lnc-FEZF2-9:2, and lnc-RP11-1035H13.3.1-2:1) identified were significantly dysregulated in further RT-PCR validation. The global “lncRNA score” calculated from the lncRNAs above also differentiated parathyroid carcinomas from adenomas.
LncRNA profiling shows distinct differentially expressed lncRNAs in parathyroid neoplasm. They may play a key role in parathyroid cancer and serve as potential biomarkers to distinguish parathyroid cancers from parathyroid adenomas.
Keywordsdiagnosis hyperparathyroidism lncRNAs parathyroid tumors
long non-coding RNA
Kyoto Encyclopedia of Genes and Genomes
reverse-transcription quantitative polymerase chain reaction
TJ, BJW, DXZ, and LL designed research; ZWC, GLQ, and XAY conducted acquisition of data; technical/material supports were provided by ZWC, XL, and XXD; technical/material support TJ, BJW, DXZ, and LL performed research and statistical analysis; TJ, BJW, DXZ, and LL wrote the paper; and BJW, DXZ, and LL conducted a critical revision of the manuscript.
This research was funded by Project of Science and Technology Research and Development of China Railway Corporation (No. J2017Z603), Special Project of Profession (No.201402007), and Foundation for young of Beijing Shijitan Hospital (2018-q13).
Compliance with ethical standards
Conflicts of interest
The authors declare that they have no conflict of interest.
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