Pilot in vitro and in vivo study on a mouse model to evaluate the safety of transcutaneous low-frequency electrical nerve stimulation on cervical cancer patients
Introduction and hypothesis
To clarify whether the pulse electrical field (PEF) caused by transcutaneous low-frequency nerve electrical stimulation (TENS) enhances the proliferation of cervical cancer cells, leading to recurrence and metastasis, and the effect of such a PEF on a cervical cancer mouse model.
1. In vitro experiment: SiHa cervical cancer cells treated with one session of microsecond PEFs for 30 min were divided into four groups: three experimental groups and the control group. Cell proliferation and migration were determined by CCK-8 proliferation and Transwell chamber Matrigel migration assay. 2. In vivo experiment: A mouse cancer model was established by subcutaneous implantation of SiHa cells that were then were randomly divided into the TENS group and control group. The former group received one session of TENS treatment and the control group received a sham pulse. The growth trend and tumor volume of each group were compared 28 days after PEF treatment. The proliferation and apoptosis of the tumor were determined by an immunohistochemical method.
(1) The CCK-8 proliferation assay and cell migration ability showed no difference after PEF stimulation treatment (F = 2.478, P = 0.136 > 0.05 and F = 0.364, P = 0.779). (2) Tumor growth, size and weight showed no significant difference between the two groups. (3) Expression of VEGF, CD34, caspase-3 and Ki-67 in the tumor tissue showed no significant difference between the two groups.
In vitro and in vivo experiments (mice) showed that the PEF created by TENS had no effect on the proliferation and migration of SiHa cervical cancer cells and also had no effect on the tumor growth, tumor cell apoptosis and proliferation.
KeywordsTranscutaneous low-frequency electrical stimulation Cervical cancer SiHa cell line Pulse electrical field
Pulse electrical field
Transcutaneous low-frequency electrical nerve stimulation
Lower urinary tract symptoms
Institutional review board
Analysis of variance
Transplanted SiHa cell tumors
Dulbecco’s modified Eagle’s medium
Major scientific and technological project of the Beijing Science and Technology Committee (D151100001915003). Chinese Preventive Medicine Association Research Funding Support Project (2013D-0101).
Compliance with ethical standards
Conflicts of interest
- 9.Fu Q-b, Jian-wei LU, Chen J, et al. Efficacy of biofeedback combined with electrical stimulation therapy for retention after radical hysterectomy. J Mod Urol. 2015;20(6):383–6.Google Scholar
- 10.Yu-mei L, Huan L, Ai-min X, et al. A clinical analysis about low frequency electric stimulation prevent urinary retention in 32 patients after radical hysterectomy. J Rare Uncommon Dis. 2010;17(2):25–7.Google Scholar
- 16.Weaver JC. Electroporation: a dramatic, nonthermal electric field phenomenon. In: Proceedings of the first world congress for electricity and magnetism in biology and medicine. Lake Buena Vista, Academic Press. 1992; pp. 127.Google Scholar
- 23.Li C-x, Sun C-x, Yao C-g, et al. Window effect of the microsecond electric pulse field strength. High Voltage Eng. 2011;37(2):388–93.Google Scholar
- 25.Sersa G, Cemazar M, Miklavcic D, et al. Electrochemotherapy of tumours. Radiol Oncol. 2006;40(3):163–74.Google Scholar
- 27.Chen XH,Ren ZG,Li CX,et al. Preclinical study of Locoregional therapy of hepatocellular carcinoma by bioelectric ablation with microsecond pulsed electric fields (μsPEFs). Sci Rep. 2015;5:1–10.Google Scholar