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Rheological evaluation of Laponite/alginate inks for 3D extrusion-based printing

Abstract

The 3D printing of soft materials is challenging mainly due to their rheological behavior. The 3D extrusion-based printing was correlated with the rheological properties for each stage of the process. A protocol to obtain an optimal ink was defined and Laponite/alginate mixtures were analyzed. All mixtures exhibited a pronounced shear-thinning behavior. Higher alginate concentrations partially hindered the rheology modifier effect of Laponite. The filament formation during extrusion and good printability were observed for Laponite concentrations of at least 5 wt%. The optimal ink was defined as a function of the viscosity profile, the filament formation ability, the flow-point and the solid-like/liquid-like behaviors. The viscosity recovery test demonstrated an instant structure recovery for the optimal ink, which did not present shear rate dependence. Jointly, an extrusion-based modular printer head was developed and tested to be compatible with open source 3D printers. Finally, the 3D printed gels were crosslinked to obtain single (SN) and double network (DN) hydrogels. In these latter, a second network precursor of poly(acrylamide) was used. As established, the rheological characterizations constitute a powerful tool to analyze the printability of soft materials.

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References

  1. 1.

    Cummins HZ (2007) Liquid, glass, gel: the phases of colloidal Laponite. J Non-Cryst Solids 353(41–43):3891

  2. 2.

    Perkins R, Brace R, Matijević E (1974) Colloid and surface properties of clay suspensions. I. Laponite CP. J Colloid Interface Sci 48(3):417

  3. 3.

    Ghadiri M, Chrzanowski W, Lee W, Fathi A, Dehghani F, Rohanizadeh R (2013) Physico-chemical, mechanical and cytotoxicity characterizations of Laponite®;/alginate nanocomposite. Appl Clay Sci 85:64

  4. 4.

    Ertesvåg H, Valla S (1998) Biosynthesis and applications of alginates. Polym Degrad Stab 59(1-3):85

  5. 5.

    Fu S, Thacker A, Sperger DM, Boni RL, Buckner IS, Velankar S, Munson EJ, Block LH (2011) Relevance of rheological properties of sodium alginate in solution to calcium alginate gel properties. AAPS PharmSciTech 12(2):453

  6. 6.

    Dávila JL, d’Ávila MA (2017) Laponite as a rheology modifier of alginate solutions: physical gelation and aging evolution. Carbohydr Polym 157:1

  7. 7.

    Malda J, Visser J, Melchels FP, Jüngst T, Hennink WE, Dhert WJ, Groll J, Hutmacher DW (2013) 25th anniversary article: engineering hydrogels for biofabrication. Adv Mater 25(36):5011

  8. 8.

    Barry RA, Shepherd RF, Hanson JN, Nuzzo RG, Wiltzius P, Lewis JA (2009) Direct-write assembly of 3D hydrogel scaffolds for guided cell growth. Adv Mater 21(23):2407

  9. 9.

    Duan B, Hockaday LA, Kang KH, Butcher JT (2013) 3D bioprinting of heterogeneous aortic valve conduits with alginate/gelatin hydrogels. J Biomed Mater Res, Part A 101(5):1255

  10. 10.

    Bakarich SE, Panhuis MIH, Beirne S, Wallace GG, Spinks GM (2013) Extrusion printing of ionic–covalent entanglement hydrogels with high toughness. J Mater Chem B 1(38):4939

  11. 11.

    Larsen BE, Bjørnstad J, Pettersen EO, Tønnesen HH, Melvik JE (2015) Rheological characterization of an injectable alginate gel system. BMC Biotechnol 15(1):1

  12. 12.

    Boere KW, Blokzijl MM, Visser J, Linssen JEA, Malda J, Hennink WE, Vermonden T (2015) Biofabrication of reinforced 3D-scaffolds using two-component hydrogels. J Mater Chem B 3(46):9067

  13. 13.

    Xavier JR, Thakur T, Desai P, Jaiswal MK, Sears N, Cosgriff-Hernandez E, Kaunas R, Gaharwar AK (2015) Bioactive nanoengineered hydrogels for bone tissue engineering: a growth-factor-free approach. ACS Nano 9(3):3109

  14. 14.

    Ahlfeld T, Cidonio G, Kilian D, Duin S, Akkineni A, Dawson J, Yang S, Lode A, Oreffo R, Gelinsky M (2017) Development of a clay based bioink for 3D cell printing for skeletal application. Biofabrication 9(3):034103

  15. 15.

    Bhattacharjee T, Zehnder SM, Rowe KG, Jain S, Nixon RM, Sawyer WG, Angelini TE (2015) Writing in the granular gel medium. Sci Adv 1(8):e1500655

  16. 16.

    Hinton TJ, Jallerat Q, Palchesko RN, Park JH, Grodzicki MS, Shue HJ, Ramadan MH, Hudson AR, Feinberg AW (2015) Three-dimensional printing of complex biological structures by freeform reversible embedding of suspended hydrogels. Sci Adv 1(9):e1500758

  17. 17.

    Markstedt K, Mantas A, Tournier I, Martiínez Ávila H, Hägg D, Gatenholm P (2015) 3D bioprinting human chondrocytes with nanocellulose–alginate bioink for cartilage tissue engineering applications. Biomacromolecules 16(5):1489

  18. 18.

    Yang F, Tadepalli V, Wiley BJ (2017) 3D printing of a double network hydrogel with a compression strength and elastic modulus greater than those of cartilage. ACS Biomater Sci Eng 3(5):863

  19. 19.

    Nonoyama T, Gong J (2015) Double-network hydrogel and its potential biomedical application: a review. Proc Inst Mech Eng H J Eng Med 229(12):853

  20. 20.

    Shin H, Olsen BD, Khademhosseini A (2012) The mechanical properties and cytotoxicity of cell-laden double-network hydrogels based on photocrosslinkable gelatin and gellan gum biomacromolecules. Biomaterials 33(11):3143

  21. 21.

    Peak CW, Stein J, Gold K, Gaharwar AK (2017) Nanoengineered colloidal inks for 3D bioprinting. Langmuir 34(3):917

  22. 22.

    Dávila JL, Freitas MS, Neto PI, Silveira ZC, Silva JVL, d’Ávila MA (2016) Software to generate 3-D continuous printing paths for the fabrication of tissue engineering scaffolds. Int J Adv Manuf Technol 84(5):1671

  23. 23.

    Yu F, Zhang F, Luan T, Zhang Z, Zhang H (2014) Rheological studies of hyaluronan solutions based on the scaling law and constitutive models. Polymer 55(1):295

  24. 24.

    Morrison FA (2001) Understanding rheology. Oxford University Press, Oxford

  25. 25.

    Bakarich SE, Gorkin R III, in het Panhuis M, Spinks GM (2014) Three-dimensional printing fiber reinforced hydrogel composites. ACS Appl Mater Interfaces 6(18):15998

  26. 26.

    Macosko CW (1994) Rheology: principles, measurements, and and applications. Wiley-VCH, New York

  27. 27.

    Li P, Dai YN, Zhang J, Wang AQ, Wei Q (2008) Chitosan-alginate nanoparticles as a novel drug delivery system for nifedipine. Int J Biomed Sci: IJBS 4(3):221

  28. 28.

    Kanti P, Srigowri K, Madhuri J, Smitha B, Sridhar S (2004) Dehydration of ethanol through blend membranes of chitosan and sodium alginate by pervaporation. Sep Purif Technol 40(3):259

  29. 29.

    Mahmoodi NM (2013) Magnetic ferrite nanoparticle–alginate composite: synthesis, characterization and binary system dye removal. J Taiwan Inst Chem Eng 44(2):322

  30. 30.

    Voo WP, Lee BB, Idris A, Islam A, Tey BT, Chan ES (2015) Production of ultra-high concentration calcium alginate beads with prolonged dissolution profile. RSC Adv 5(46):36687

  31. 31.

    Du J, Zhu J, Wu R, Xu S, Tan Y, Wang J (2015) A facile approach to prepare strong poly (acrylic acid)/LAPONITE®; ionic nanocomposite hydrogels at high clay concentrations. RSC Adv 5(74):60152

  32. 32.

    Jun CS, Sim B, Choi HJ (2015) Fabrication of electric-stimuli responsive polyaniline/laponite composite and its viscoelastic and dielectric characteristics. Colloids Surf A Physicochem Eng Asp 482:670

  33. 33.

    Ghadiri M, Hau H, Chrzanowski W, Agus H, Rohanizadeh R (2013) Laponite clay as a carrier for in situ delivery of tetracycline. RSC Adv 3(43):20193

  34. 34.

    Sudha JD, Pich A, Reena VL, Sivakala S, Adler HJP (2011) Water-dispersible multifunctional polyaniline-laponite-keggin iron nanocomposites through a template approach. J Mater Chem 21(41):16642

  35. 35.

    Li Q, Siddaramaiah B, Kim NH, Heo SB, Lee JH (2008) Novel PAAm/Laponite clay nanocomposite hydrogels with improved cationic dye adsorption behavior. Compos Part B 39(5):756

  36. 36.

    Şolpan D, Torun M, Güven O (2008) The usability of (sodium alginate/acrylamide) semi-interpenetrating polymer networks on removal of some textile dyes. J Appl Polym Sci 108(6):3787

  37. 37.

    Lim BC, Singu BS, Hong SE, Na YH, Yoon KR (2016) Synthesis and characterization nanocomposite of polyacrylamide-rGO-Ag-PEDOT/PSS hydrogels by photo polymerization method. Polym Adv Technol 27(3):366

  38. 38.

    Cai WR, Zhang GY, Song T, Zhang XJ, Shan D (2016) Cobalt hexacyanoferrate electrodeposited on electrode with the assistance of laponite: the enhanced electrochemical sensing of captopril. Electrochim Acta 198:32

  39. 39.

    Zhang H, Zhai D, He Y (2014) Graphene oxide/polyacrylamide/carboxymethyl cellulose sodium nanocomposite hydrogel with enhanced mechanical strength: preparation, characterization and the swelling behavior. RSC Adv 4(84):44600

  40. 40.

    Li Z, He G, Hua J, Wu M, Guo W, Gong J, Zhang J, Qiao C (2017) Preparation of γ-PGA hydrogels and swelling behaviors in salt solutions with different ionic valence numbers. RSC Adv 7(18):11085

  41. 41.

    Liu S, Li H, Tang B, Bi S, Li L (2016) Scaling law and microstructure of alginate hydrogel. Carbohydr Polym 135:101

  42. 42.

    Yang CH, Wang MX, Haider H, Yang JH, Sun JY, Chen YM, Zhou J, Suo Z (2013) Strengthening alginate/polyacrylamide hydrogels using various multivalent cations. ACS Appl Mater Interfaces 5(21):10418

  43. 43.

    Zhao X (2014) Multi-scale multi-mechanism design of tough hydrogels: building dissipation into stretchy networks. Soft Matter 10(5):672

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Correspondence to José Luis Dávila.

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Dávila, J., d’Ávila, M. Rheological evaluation of Laponite/alginate inks for 3D extrusion-based printing. Int J Adv Manuf Technol 101, 675–686 (2019) doi:10.1007/s00170-018-2876-y

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Keywords

  • Rheology
  • 3D printing
  • Laponite
  • Alginate
  • Hydrogels