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A rat model of monitoring liver allograft rejection

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Rat models are often used to study liver allograft rejection. We have established a model for rat liver allograft rejection, monitored by fine needle aspiration biopsy (FNAB), in the strain combination PVG-to-BN with a mean survival time of 37 ± 20 days. In this model, we observed acute rejection with an intense peak of lymphoid blasts and lymphocyte-dominated inflammation in the FNAB [9.1 ± 3.0 corrected increment units (CIU)], and an eventual increase in macrophages (up to 4.2 ± 4.4 CIU), together with fibrosis and parenchymal necrosis in the graft. Markers of immune activation, such as an increase in IL-2-receptor (from 1 % ± 2 % to 21 % ± 13 %) and class II (from 20 % ± 9 % to 43 % ± 13 %) expressing lymphoid cells and induction of ICAM-1 in the graft, were consistent with the overall cellular response. The FNAB correlated well with parallel graft histology. In this rat model, the atraumatic monitoring makes a close follow-up possible without having to sacrifice the experimental animals. This saves work, animals, and costs in the study of liver rejection.

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Received: 2 July 1996 Accepted: 28 October 1996

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Martelius, T., Mäkisalo, H., Höckerstedt, K. et al. A rat model of monitoring liver allograft rejection. Transpl Int 10, 103–108 (1997).

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  • Key words Liver transplantation
  • rat model
  • Rat
  • liver transplantation
  • fine needle aspiration
  • Fine needle aspiration
  • liver
  • rat
  • Rejection
  • liver
  • rat