Can Local Enhancement in Earthworms Affect the Outcome of the Standard Earthworm Avoidance Test?
Earthworms exhibit clumping behaviour in and out of the soil. However, it remains unknown if such social behaviour ultimately influences the outcome of ecotoxicological experiments in the laboratory. We performed several overnight avoidance tests to determine whether social behaviour (i.e., local enhancement) is a factor in pollution avoidance behaviour in the earthworm Eisenia fetida. The results showed that there was no clear influence of social behaviour on the choice or avoidance of Cd contaminated soils, although we suspect that 50 mg Cd/kg might not have been high enough to elicit a significant avoidance response. Nevertheless, when offered a choice between clean undisturbed soil and previously inhabited soil, the worms preferred the previously inhabited soil (p < 0.01). While the level of metal pollution investigated in this study did not disrupt or help predict social dynamics, local enhancement, perhaps driven by some sort of habitat imprinting, was successfully documented in Eisenia fetida.
KeywordsAvoidance behaviour Avoidance test Cadmium Earthworms Ecotoxicology
- Demuynck S, Lebel A, Grumiaux F et al (2016) Comparative avoidance behaviour of the earthworm Eisenia fetida towards chloride, nitrate and sulphate salts of Cd, Cu and Zn using filter paper and extruded water agar gels as exposure media. Ecotoxicol Environ Saf 129:66–74. https://doi.org/10.1016/j.ecoenv.2016.03.013 CrossRefGoogle Scholar
- Dou J, Hu S (2014) Individual and combined toxicity of chromium and cadmium on the earthworm Eisenia fetida. Biotechnol Indian J 10:9737–9742Google Scholar
- European Economic Community (1984) Council directive 79/831/EEC Annex V, part C. Method for the determination of ecotoxicity. Level 1. Earthworms: artificial soil test. Commission of the European Communities. DGXI/128/82 Rev. 5Google Scholar
- International Organization for Standardization (2008) ISO 17512-1 - Soil quality – avoidance test for determining the quality of soils and effects of chemicals on behaviour – part 1: test with earthworms (Eisenia foetida and Eisenia andrei). 12:27Google Scholar
- International Organization for Standardization (2012a) ISO 11268-1:2012 – Soil quality – effects of pollutants on earthworms – part 1: determination of acute toxicity to Eisenia fetida/Eisenia andrei. 18Google Scholar
- International Organization for Standardization (2012b) ISO 11268-2:2012 – Soil quality – effects of pollutants on earthworms – part 2: determination of effects on reproduction of Eisenia fetida/Eisenia andrei Google Scholar
- Machado RC, Amaral CDB, Schiavo D et al (2017) Complex samples and spectral interferences in ICP-MS: evaluation of tandem mass spectrometry for interference-free determination of cadmium, tin and platinum group elements. Microchem J 130:271–275. https://doi.org/10.1016/j.microc.2016.09.011 CrossRefGoogle Scholar
- Mathieu J, Barot S, Blouin M et al (2010) Habitat quality, conspecific density, and habitat pre-use affect the dispersal behaviour of two earthworm species, Aporrectodea icterica and Dendrobaena veneta, in a mesocosm experiment. Soil Biol Biochem 42:203–209. https://doi.org/10.1016/j.soilbio.2009.10.018 CrossRefGoogle Scholar
- Reinecke AJ, Reinecke SA (1996) The influence of heavy metals on growth and reproduction of the compost worm Eisenia fetida (Oligochaeta). Pedobiologia 40:439–449Google Scholar
- Thorpe W (1963) Learning and instinct in animals, 2nd edn. Methuen, LondonGoogle Scholar
- USEPA (1988) Protocols for short- term toxicity screening of hazardous waste sites, EPA/600/3-88/029Google Scholar
- USEPA (1996) Method 3052: microwave assisted acid digestion of siliceous and organically based matrices. https://www.epa.gov/sites/production/files/2015-12/documents/3052.pdf. Accessed 5 Oct 2019