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Silver Nanoparticles Induced Cell Apoptosis, Membrane Damage of Azotobacter vinelandii and Nitrosomonas europaea via Generation of Reactive Oxygen Species

  • Li Zhang
  • Lingli Wu
  • Yazhu Mi
  • Youbin SiEmail author
Article

Abstract

Silver nanoparticles (AgNPs) is widely used as an antibacterial agent, but the specific antibacterial mechanism is still conflicting. This study aimed to investigate the size dependent inhibition of AgNPs and the relationship between inhibition and reactive oxygen species (ROS). Azotobactervinelandii and Nitrosomonaseuropaea were exposed to AgNPs with different particles size (10 nm and 50 nm). The ROS production was measured and the results showed that the generation of ROS related to the particle size and concentrations of AgNPs. At 10 mg/L of 10 nm Ag particles, the apoptosis rate of A. vinelandii and N. europaea were 20.23% and 1.87% respectively. Additionally, the necrosis rate of A. vinelandii and N. europaea reached to 15.20% and 42.20% respectively. Furthermore, transmission electron microscopy images also indicated that AgNPs caused severely bacterial cell membrane damage. Together these data suggested that the toxicity of AgNPs depends on its particle size and overproduction of ROS.

Keywords

Silver nanoparticles Reactive oxygen species Azotobactervinelandii Nitrosomonaseuropaea Apoptosis 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China [Grant No. 41430752].

Compliance with Ethical Standards

Conflict of interest

We declare that we have no conflict of interest.

Research Involving Human Participants and/or Animals

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

128_2019_2622_MOESM1_ESM.doc (36 kb)
Supplementary file1 (DOC 35 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and EnvironmentAnhui Agricultural UniversityHefeiChina

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