Preliminary Assessment of Stress and Genotoxicity Biomarkers in Bivalve Molluscs from the Gulf of Annaba, Algeria

  • Fahima Drif
  • Cherif AbdennourEmail author
  • İbrahim Hakkı Ciğerci
  • Muhammad Muddassir Ali
  • Ouarda Mansouri
  • Mahfoud Messarah


The aim of this work is to assess biomarker responses to stresses caused by pollution of the marine environment in the Gulf of Annaba, using the bivalve mollusc Donax trunculus and Mytilus galloprovincialis. Males and females were collected during the summer from site 1 considered to be far from pollution sources (SM1 and SD1), site 2 (SM2 and SD2) exposed to a mixture of contaminants, and site 3 (SM3 and SD3) receiving the untreated sewage. The whole body was used for the determination of malonaldehyde (MDA), reduced glutathione (GSH) and DNA damage. The MDA concentration from site 2 of both species has indicated significant augmentation. The two bivalves from S2 and S3 have recorded significant decrease of GSH level, with the highest number of Comet scores. A significant site and sex effect was measured for the level of GSH and MDA, accompanied with a significant site effect for DNA damage. The genotoxicity seems correlated with the stress biomarkers and dependent on the effluent types.


Bivalves Comet scores GSH MDA D. trunculus M. galloprovincialis 



We would like to thank The National Research Fund, Algeria (FNR) for financial support to the Laboratory of Animal Ecophysiology (FNR/LAB0033/2016).


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Fahima Drif
    • 1
  • Cherif Abdennour
    • 2
    Email author
  • İbrahim Hakkı Ciğerci
    • 3
  • Muhammad Muddassir Ali
    • 4
  • Ouarda Mansouri
    • 2
  • Mahfoud Messarah
    • 5
  1. 1.Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre et de l’UniversUniversité 8 Mai 1945 GuelmaGuelmaAlgeria
  2. 2.Laboratory of Animal Ecophysiology, Department of Biology, Faculty of SciencesUniversity Badji Mokhtar-AnnabaAnnabaAlgeria
  3. 3.Laboratory of Molecular Biology and Genetic, Faculty of Arts and SciencesUniversity Afyon KocatepeAfyonkarahisarTurkey
  4. 4.Institute of Biochemistry and BiotechnologyUniversity of Veterinary and Animal SciencesLahorePakistan
  5. 5.Laboratory of Biochemistry and Environmental Toxicology, Department of Biochemistry, Faculty of SciencesUniversity Badji Mokhtar-AnnabaAnnabaAlgeria

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