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DNA Damage and Immunological Responses in the Whiteleg Shrimp (Litopenaeus vannamei) Exposed to Sublethal Levels of Mercury

  • Sarahi Roos-Muñoz
  • Domenico Voltolina
  • Marisela Aguilar-Juárez
  • Selene Abad-Rosales
  • Juan C. Bautista-Covarrubias
  • M. Isaura Bañuelos-Vargas
  • Martín F. Soto-Jiménez
  • Martín G. Frías-Espericueta
Article

Abstract

Litopenaeus vannamei juveniles were exposed to sublethal levels (2.33–18.03 µg/L) of inorganic mercury. Time of exposure (0, 24 and 168 h) was a source of DNA damage. Mean comet tail length not changed significantly with mercury concentrations and exposure time, and this parameter cannot be used to assess DNA damage in this shrimp. Total hemocyte count showed a trend to decrease according to the increase of mercury concentrations, although no significant difference between treatments with mercury was observed. The phenoloxidase (PO) activity was not influenced by the time of exposure. At the end of the experiment, the PO in organisms exposed to 18.03 µg/L was different from the control. The time of exposure has a more important influence in superoxide dismutase than the concentration of mercury. According to these results, a suitable criterion of water quality for long-term exposure of L. vannamei should be lower than 2 µg/L of mercury.

Keywords

Mercury Litopenaeus vannamei Genotoxicity Immune responses Hemocytes 

Notes

Acknowledgements

This work was supported by a CONACYT scholarship to the first author and by grants PROFAPI UAS (2015/004, 2015/103), PROMEP UAS (PTC-105.DSA/103.5/14/10808), PRODEP (CANE year 3) and CONACYT INFRA 2012-01-188029.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Sarahi Roos-Muñoz
    • 1
  • Domenico Voltolina
    • 2
  • Marisela Aguilar-Juárez
    • 3
  • Selene Abad-Rosales
    • 4
  • Juan C. Bautista-Covarrubias
    • 5
  • M. Isaura Bañuelos-Vargas
    • 3
  • Martín F. Soto-Jiménez
    • 6
  • Martín G. Frías-Espericueta
    • 3
  1. 1.Posgrado en Ciencias en Recursos AcuáticosUniversidad Autónoma de SinaloaMazatlánMexico
  2. 2.Centro de Investigaciones Biológicas del NoroesteLaboratorio UAS-CIBNORMazatlánMexico
  3. 3.Facultad de Ciencias del MarUniversidad Autónoma de SinaloaMazatlánMexico
  4. 4.CIAD, Unidad MazatlánMazatlánMexico
  5. 5.Unidad Académica Escuela Nacional de Ingeniería PesqueraUniversidad Autónoma de NayaritSan BlasMexico
  6. 6.Instituto de Ciencias del Mar y LimnologíaUniversidad Nacional Autónoma de MéxicoMazatlánMexico

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