Bioaccumulation of Copper and Zinc and the Effects on Antioxidant Enzyme Activities in the Liver of Acipenser stellatus (Pallas, 1771)

  • Daniela Vasile
  • Gisela Gaina
  • Lucian Cristian Petcu
  • Dragomir Coprean
  • Lucica TofanEmail author
  • Anca Dinischiotu


Although water pollution by metals in the Danube River is considered high, little is known about its impact on sturgeons. In this regard, the aim of this study was to investigate the bioaccumulation of copper and zinc as well as their effects on antioxidant enzymes activities in the liver of Acipenser stellatus. The fish were exposed for 7 and 14 days, to two concentrations of copper and zinc (10% and 25% of LC50 96 h), previously determined as 0.54 mg/L Cu2+ and, 34.22 mg/L Zn2+ respectively. The enzymatic responses of A. stellatus varied greatly depending on metal type, concentration and time. Significant bioaccumulation of the two metals was recorded. Even though the water hardness used in the experiment is known to offer a clear protection against metal contamination, stellate sturgeon remains a sensitive species in the face of metal toxicity.


Acipenser stellatus Copper and zinc Antioxidant enzymes Bioaccumulation 



We bring our gratitude to Prof. Ph.D. Patriche Neculai, director of Institute of Research and Development for Aquaculture, Fishing and Aquatic Ecology from Galati and to Ph.D. Eng. Magdalena Tenciu for all their scientific and technical support.


  1. Aebi H (1974) Catalase. In: Bergmeyer UH (ed) Methods of enzymatic analysis. Verlag Chemie/Academic Press, Weinheim/New York, pp 673–684CrossRefGoogle Scholar
  2. Atli G, Canli M (2007) Enzymatic responses to metal exposures in a freshwater fish Oreochromis niloticus. Comp Biochem Physiol Part C, 145:282–287Google Scholar
  3. Atli G, Canli M (2008) Responses of metallothionein and reduced glutathione in a freshwater fish Oreochromis niloticus following metal exposures. Environ Toxicol Pharmacol 25:33–38CrossRefGoogle Scholar
  4. Atli G, Canli M (2010) Response of antioxidant system of freshwater fish Oreochromis niloticus to acute and chronic metal (Cd, Cu, Cr, Zn, Fe) exposures. Ecotoxicol Environ Saf 73:1884–1889CrossRefGoogle Scholar
  5. Bacalbasa-Dobrovici N (1997) Endangered migratory sturgeons of the lower Danube River and its delta. Environ Biol Fishes 48:201–207CrossRefGoogle Scholar
  6. Beutler E (1984) Glutathione peroxidase. In: Beutler E (ed) Red cell metabolism. A manual of biochemical methods, 3rd edn. Grune and Stratton, Inc., OrlandoGoogle Scholar
  7. Bird G, Brewer P, Macklin M (2010) Management of the Danube drainage basin: implications of contaminant-metal dispersal for the implementation of the EU Water Framework Directive. Int J River Basin Manag 8(1):63–78CrossRefGoogle Scholar
  8. Brucka-Jastrzebska E, Kawczuga D, Rajkowska M, Protasowicki M (2009) Levels of microelements (Cu, Zn, Fe) and macroelements (Mg, Ca) in freshwater fish. J Elementol 14(3):437–447Google Scholar
  9. Cankay AM, Sisecioglu M, Ciftci M, Ozdemir H (2011) Effects of some metal ions on trout liver glucose-6-phosphate dehydrogenase. Res J Environ Toxicol 5(6):385–391CrossRefGoogle Scholar
  10. Crupkin AC, Menone ML (2013) Changes in the activities of glutathione s- transferase, glutathione reductase and catalase after exposure to different concentrations of cadmium in Australoheros facetus (Cichlidae, Pisces). Ecotoxivcol Environ Contam 8(1):21–25Google Scholar
  11. Doering J, Beitel S, Eisner B, Heide T, Hollert H, Giesy J, Hecker M, Wiseman S (2015) Identification and response to metals of metallothionein in two ancient fishes: white sturgeon (Acipenser transmontanus) and lake sturgeon (Acipenser fulvescens). Comp Biochem Physiol Part C 171:41–48Google Scholar
  12. Goldberg DM, Spooner RJ (1983) Glutathione reductase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Verlag Chemie, WeinheimGoogle Scholar
  13. Grossel M, Wood CM (2002) Copper uptake across rainbow trout gills: mechanisms of apical entry. J Exp Biol 205:1179–1188Google Scholar
  14. Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139Google Scholar
  15. Handy RD, Eddy FB, Baines H (2002) Sodium-dependent copper uptake across epithelia:a review of rationale with experimental evidence from gill and intestine. Biochem Biophys Acta 1566(1–2):104–115CrossRefGoogle Scholar
  16. Hogstrand C, Wood C (1995) Mechanisms for zinc acclimatization in freshwater rainbow trout. Maine Environ Res 39:131–135CrossRefGoogle Scholar
  17. ICPDR (2015) Joint Danube Survey 3. A comprehensive analysis of danube water quality. International Commission for the Protection of the Danube River, ViennaGoogle Scholar
  18. IUCN (2010) The IUCN (International Union for Conservation of Nature) red list of threatened speciesGoogle Scholar
  19. Jaric I, Gessner J (2012) Analysis of publications on sturgeon research between 1996 and 2010. Scientometrics 90:715–735CrossRefGoogle Scholar
  20. Jaric I, VIsnjic-Jeftic Z, Cvijanovic G, Gacic Z, Jovanovic L, Skoric S, Lenhardt M (2011) Determination of differential heavy metal and trace element accumulation in liver, gills, intestine and muscle of starlet (Acipenser ruthenus) from the Danube River in Serbia by ICP-OES. Microchem J 98:77–81CrossRefGoogle Scholar
  21. Langan LM, Harper GM, Owen SF, Purcell WM, Jackson SK, Jha AN (2017) Application of the rainbow trout derived intestinal cell line (RTgutGC) for ecotoxicological studies: molecular and cellular responses following exposure to copper. Ecotoxicology 26(8):1117–1133CrossRefGoogle Scholar
  22. Little E, Calfee R, Linder G (2012) Toxicity of copper to early life stage Kootenai River white sturgeon, Columbia River white sturgeon and rainbow trout. Arch Environ Contam Toxicol 63:400–408CrossRefGoogle Scholar
  23. Lohr GW, Waller HD (1974) Glucoso-6-phosphate dehydrogenase. In: Bergmeyer UH (ed) Methods of enzymatic analysis. Verlag Chemie/Academic Press, Weinheim/New YorkGoogle Scholar
  24. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin-Phenol reagents. J Biol Chem 193:265–275Google Scholar
  25. Paoletti F, Aldinucci D, Mocali A, Caparrini A (1986) A sensitive spectrophotometric method for the determination of superoxide dismutase activity in tissue extracts. Anal Biochem 154:536–541CrossRefGoogle Scholar
  26. Poleksic V, Lenhardt M, Jaric I, Djordjevic D, Gacic Z, Cvijanovic G, Raskovic B (2010) Liver, gills and skin histopathology and heavy metal content of the Danube starlet (Acipenser ruthenus Linnaeus, 1758). Environ Toxicol Chem 29(3):515–521CrossRefGoogle Scholar
  27. Popa P, Patriche N, Mocanu R, Sârbu C (2001) Quality of aquatic environment—control methods and interpretation. Ceres Publishing, BucharestGoogle Scholar
  28. Sanchez W, Palluel O, Meunier L, Coquery M, Porcher JM, Selim A (2005) Copper induced oxidative stress in three-spined stickleback: relationship with hepatic metal levels. Environ Toxicol Pharmacol 19(1):177–183CrossRefGoogle Scholar
  29. Srikanth K, Pereira E, Duarte AC, Ahmad I (2013) Glutathione and its dependent enzymes’ modulatory responses to toxic metals and metalloids in fish—a review. Environ Sci Pollut Res 20:2133–2149CrossRefGoogle Scholar
  30. Trenzado C, Hidalgo MC, Garcia-Gallego M, Morales AE, Furne M, Domezain A, Domezain J, Sanz A (2006) Antioxidant enzymes and lipid peroxidation in sturgeon Acipenser naccarii and trout Oncorhynchus mykiss. A comparative study. Aquaculture 254:758–767CrossRefGoogle Scholar
  31. Tudor M, Teodorof L, Burada A, Tudor M, Ibram O, Despina C (2016) Long-term nutrients and heavy metals concentration dynamics in aquatic ecosystems of Danube Delta. Sci Ann Danube Delta Institute 22:149–156Google Scholar
  32. Van der Oost R, Beyer J, Vermeulen N (2003) Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environ Toxicol Pharmacol 13:57–149CrossRefGoogle Scholar
  33. Vardy D, Tompsett A, Sigurdson J, Doering J, Zhang X, Giesy J, Hecker M (2011) Effects of subchronic exposure of early life stages of white sturgeon (Acipenser transmontanus) to copper, cadmium and zinc. Environ Toxicol Chem 30:2487–2505CrossRefGoogle Scholar
  34. Vasile D, Tenciu M, Patriche N, Costache M, Coprean D, Dinischiotu A, Tofan L (2015) The acute toxicity of copper and zinc on the protected stellate sturgeon juveniles (Acipenser stellatus Pallas, 1771). Carpathian J Earth Environ Sci 10(1):101–106Google Scholar
  35. Vecsei P, Peterson D, Suciu R, Artyukhin E (2007) Threatened fishes of the world, Acipenser stellatus, Pallas, 1771 (Acipenseridae). Environ Biol Fishes 78:211–212CrossRefGoogle Scholar
  36. Zahedi S, Mirvaghefi A, Amiri M, Rafiee G, Hedayati M, Makhdoomi C, Dagesaraki Z, Sahebi M (2012) The effects of copper and cadmium exposure on biochemical factors of plasma and liver in beluga sturgeon Huso huso (Linnaeus, 1758). J Fish Iran J Nat Res 65(3):271–282Google Scholar

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Authors and Affiliations

  1. 1.Faculty of Natural and Agricultural Sciences“Ovidius” University ConstantaConstanţaRomania
  2. 2.Department of Biochemistry and Molecular BiologyUniversity of BucharestBucharestRomania

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