Determination of Formaldehyde in Water Samples by High-Performance Liquid Chromatography with Methyl Acetoacetate Derivatization

  • Kenji YoshikawaEmail author
  • Yusuke Oshima
  • Ayaka Inagaki
  • Akio Sakuragawa


A high-performance liquid chromatography method with methyl acetoacetate derivatization via the Hantzsch reaction was developed for the analysis of formaldehyde (HCHO) in several water samples. Under optimized conditions, HCHO was detected within 4 min and was not affected by excessive derivatization reagents. The calibration curve constructed from the peak height of HCHO was linear, with a correlation coefficient of 0.9998. The relative standard deviation of the peak height from ten replicates was 0.29%. The detection and quantitative limits were 0.96 µg/L and 3.16 µg/L, respectively. A recovery test of HCHO was performed to compare the developed method with the official analysis method (DNPH method). The developed method was used to determine the HCHO levels in several water samples (tap water, river water, and waste water).


Formaldehyde High-performance liquid chromatography Hantzsch reaction Derivatization Methyl acetoacetate Water samples 



We would like to thank Editage ( for English language editing.


No funding received for this study.

Compliance with Ethical Standards

Conflict of Interests

The authors declare that they have no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Kenji Yoshikawa
    • 1
    Email author
  • Yusuke Oshima
    • 1
  • Ayaka Inagaki
    • 1
  • Akio Sakuragawa
    • 1
  1. 1.Department of Materials and Applied Chemistry, College of Science and TechnologyNihon UniversityTokyoChina

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