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Effects of Petroleum Hydrocarbon Contaminated Soil on Germination, Metabolism and Early Growth of Green Gram, Vigna radiata L.

Abstract

The objective of the present study was to evaluate effects of petroleum hydrocarbon contaminated soil on the leguminous plant, Vigna radiata L. Seed germination, metabolism and early growth performance of V. radiata L. were studied as parameters by applying a combined approach. The employed combined method which included microcalorimetry and analysis of the root cross section revealed dose dependent effects of petroleum hydrocarbon contaminated soil on V. radiata L. for most parameters. Although significant reductions in measured parameters were observed even at low total petroleum hydrocarbon (TPH) levels such as 1 % and 1.5 %, calculated inhibitions, IC50 values and metabolic heat emission-time curves inferred that substantial negative effects can be expected on V. radiata L. in soils with comparatively high contamination levels, such as 2.5 % TPH and higher.

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Acknowledgments

This work was supported in part by grants from the International Joint Key Project from Chinese Ministry of Science and Technology (2010DFA12780), International Joint Key Project from National Natural Science Foundation of China (40920134003), National Natural Science Foundation of China (41273092) and National Outstanding Youth Research Foundation of China (40925010). KM acknowledges the financial support by the Chinese Government Scholarship through Chinese Scholarship Council.

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Correspondence to Jun Yao.

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Masakorala, K., Yao, J., Chandankere, R. et al. Effects of Petroleum Hydrocarbon Contaminated Soil on Germination, Metabolism and Early Growth of Green Gram, Vigna radiata L.. Bull Environ Contam Toxicol 91, 224–230 (2013). https://doi.org/10.1007/s00128-013-1042-3

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Keywords

  • Petroleum contaminated soil
  • Vigna radiata L.
  • Metabolism
  • Microcalrimetry