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Determination of T-2 and HT-2 Toxins in Commodities and Feed in Croatia


Data on the occurrence of T-2 and HT-2 toxins in commodities and feed in Croatia and in Europe are very limited. In the present study, 50 feed and commodity samples tested for the presence of these mycotoxins yielded highest concentrations in cattle feed (67.68 ng/g) and oat samples (32.94 ng/g). In the future, screening should include greater numbers of feed and commodity samples to shed more light on the presence of T-2 and HT-2 toxins.

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  1. Bennett JW, Klich M (2003) Mycotoxins. Clin Microbiol Rev 16:497–516

  2. Binder EM, Tan LM, Chin LJ, Handl J, Richard J (2007) Worldwide occurrence of mycotoxins in commodities, feeds and feed ingredients. Anim Feed Sci Technol 137:265–282

  3. Boermansn HJ, Leung MCK (2007) Mycotoxins and the pet food industry: toxicological evidence and risk assessment. Int J Food Microbiol 119:95–102

  4. Commission Recommendation 2006/576/EC of 17 August 2006 on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 and fumonisins in products intented for animal feeding. Official Journal of the European Union L 229/7

  5. Creppy EE (2002) Update of survey, regulation and toxic effects of mycotoxins in Europe. Toxicol Lett 127:19–28

  6. Fuchs E, Binder EM, Heidler D, Krska R (2002) Structural characterization of metabolites after the microbial degradation of type A trichothecenes by the bacterial strain BBSH 797. Food Addit Contam 19:379–386

  7. Glenn AE (2007) Mycotoxigenic Fusarium species in animal feed. Anim Feed Sci Technol 137:213–240

  8. Labuda R, Parich A, Berthiller F, Tančinova D (2005) Incidence of trichothecenes and zearalenone in poultry feed mixtures from Slovakia. Int J Food Microbiol 105:19–25

  9. Langseth W, Rundberget T (1999) The occurrence of HT-2 and other trichothecenes in Norwegian cereals. Mycopathologia 147:157–165

  10. Lincy SV, Latha R, Chandrashekar A, Manonnami HK (2008) Detection of toxigenic fungi and quantification of type A trichothecene levels in some food and feed materials from India. Food Control 19:962–966

  11. Mankevičiene A, Butkutė B, Dabkevičius Z, Supronienė S (2007) Fusarium mycotoxins in Lithuanian cereals from the 2004–2005 harvests. Ann Agric Environ Med 14:103–107

  12. Meister U (2008) Analysis of T-2 and HT-2 toxins in oat and other cereals by means of HPLC with fluorescence detection. Mycotoxin Res 24:31–39

  13. Milanez TV, Valente-Soares LM, Baptista GG (2006) Occurrence of trichothecene mycotoxins in Brazilian corn-based food products. Food Control 17:293–298

  14. Morgavi DP, Riley RT (2007) An historical overview of field disease outbreaks known or suspected to be caused by consumption of feeds contaminated with Fusarium toxins. Anim Feed Sci Technol 137:201–212

  15. Official Gazzete of the Republic of Croatia 118/2007

  16. Peraica M, Radić B, Lucić A, Pavlović M (1999) Toxic effects of mycotoxin in humans. Int J Public Health 77:754–767

  17. Richard JL (2007) Some major mycotoxins and their mycotoxicoses–an overview. Int J Food Microbiol 119:3–10

  18. Schollenberger M, Müller HM, Rüfle M, Suchy S, Plank S, Drochner W (2005) Survey of Fusarium toxins in foodstuffs of plant origin marketed in Germany. Int J Food Microbiol 97:317–326

  19. Scientific Committee on Food (2002). Opinion of the Scientific Commitee on Food on Fusarium toxins. Part 6: Group evaluation on T-2 and HT-2 toxin, nivalenol and deoxynivalenol

  20. Sokolović M, Šimpraga B (2006) Survey of trichothecene mycotoxins in grains and animal feed in Croatia by thin layer chromatography. Food Control 17:733–740

  21. Ueno Y, Nakayama K, Ishii K, Tashiro F, Minoda Y, Omori T, Komagata K (1983) Metabolism of T-2 toxin in Curtobacterium sp. Strain 114–2. Appl Envion Microbiol 46:120–127

  22. Van Egmond HP (2004) Natural toxins: risks, regulations and the analytical situation in Europe. Anal Bioanal Chem 378:1152–1160

  23. Young JC, Zhou T, Yu H, Zhu H, Gong J (2007) Degradation of trichothecene mycotoxins by chicken intestinal microbes. Food Chem Toxicol 45:136–143

  24. Zhou T, He J, Gong J (2008) Microbial transformation of trichothecene mycotoxins. World Mycotoxin J 1:23–30

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Correspondence to Ana Vulić.

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Vulić, A., Pleadin, J. & Perši, N. Determination of T-2 and HT-2 Toxins in Commodities and Feed in Croatia. Bull Environ Contam Toxicol 86, 294–297 (2011). https://doi.org/10.1007/s00128-011-0219-x

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  • T-2 HT-2
  • Feed
  • Commodities