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Isolation, Identification and Degradation Characterization of a p-Chloroaniline Degrading Strain

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Abstract

A bacterium (strain ycsd02) was isolated from rhizosphere soil in phragmites wetland using the enrichment culture contaminated with p-Chloroaniline (p-CA). Morphology, physio-biochemical characteristics and phylogenetic analysis based on 16S rDNA sequence indicated that the shape of the single cell is pole, which clearly belongs to Bacillus licheniformis. The optimal conditions for satisfactory degradation of p-CA were also studied. A dynamic test indicated that the biodegradation had no lag phase at concentrations from 0 to 20 mg/L in minimal medium culture fluids at vaccination of 15%, pH 7.0 and 32°C, and the growth of strain ycsd02 cells met the first-order kinetics.

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Acknowledgment

This study was supported by the National Natural Science Fund of China (No.20607018), the Natural Science Fund of Jiangsu Province (No.BK2009171), and Innovation Fund for Technology Based Firms of Department of Science and Technology of China (No.09C26213201069) and Jiangsu Province (No. BC2007132).

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Correspondence to Cheng Ding.

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Ding, C., Li, Z. & Yan, J. Isolation, Identification and Degradation Characterization of a p-Chloroaniline Degrading Strain. Bull Environ Contam Toxicol 86, 454–459 (2011). https://doi.org/10.1007/s00128-011-0196-0

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Keywords

  • p-Chloroaniline
  • Identification
  • Degradation
  • 16S rDNA