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Biosorption of Pb2+ by Saccharomyces Cerevisiae in Static and Dynamic Adsorption Tests

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This paper demonstrates the Pb2+ adsorption capacity and adsorption rate of Saccharomyces cerevisiae by both static and dynamic testing to verify its feasibility as a heavy metal bio-absorbent in wastewater treatment. The static testing was divided into two parts. First, we tested S. cerevisiae by itself, and then we tested immobilized S. cerevisiae. In static testing of the non-immobilized S. cerevisiae, the Pb2+ adsorption capacity and adsorption rate increased up to 6.52 mg/g and 52.94%, respectively, with time. After immobilization, the Pb2+ adsorption capacity and adsorption rate reached 10 mg/g and 80%, respectively. In dynamic testing, the optimal saturated adsorption capacity of immobilized S. cerevisiae for Pb2+ was 6.64 mg/g. In addition to the static and dynamic testing of adsorption capacity and rate, we used SEM imaging to analyze the mechanics of adsorption, and the images showed that the cell wall played the major roll in Pb2+ adsorption.

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  1. Churchill SA, Walters JV, Churchill PF (1995) Sorption of heavy metals by prepared bacterial cell surfaces. J Environ Eng 121(10):706–711

  2. Gabaldon C, Marzal P, Ferrer J, Seco A (1996) Single and competitive adsorption of Cd and Zn onto a granular activated carbon. Wat Res 30:3050–3060

  3. Hu JJ, Zhou QY (1988) Environmental engineering microbiology. Higher Education Press, Bei Jing (in Chinese)

  4. Iqbal M, Saeed A, Edyvean RGJ, Sullivan BO, Styring P (2005) Production of fungal biomass immobilized loofa sponge (FBILS)-discs for the removal of heavy metal ions and chlorinated compounds from aqueous solution. Biotechnol Lett 27:1319–1323

  5. Jansson-Charrier M, Guibal E, Roussy J, Delanghe B, Le Cloirec P (1996) Vanadium (IV) sorption by chitosan: kinetics and equilibrium. Wat Res 30:456–475

  6. Laube V, Ramamoorthy S, Kushner DJ (1979) Mobilization and accumulation of sediment bound heavy metals by algae. Bull Environ Contamin Toxicol 21:763–770

  7. Lopez A, Lazaro N, Priego JM, Marques AM (2000) Effect of pH on the biosorption of nickel and other heavy metals by Pseudomonas fluorescens 4F39. J Industrial Microbiol Biotechnol 24:146–151

  8. Machado R, Santos C, Joana Neiva Correia M, Carvalho JR (2003) Biosorption of copper by grape stalks and pine bark biomasses. European J Mineral Process Environ Protection 3(1):108–118

  9. Mann H (1990) Biosorption of heavy metals by bacterial biomass. Biosorption of heavy metals. CRC Press, Boca Raton, pp 93–137

  10. Ridvan S, Nalan Y, Adil D (2003) Biosorption of cadmium, lead, mercury and arsenic ions by the fungus Penicillium purpurogenum. Sep Sci Technol 38(9):2039–2053

  11. Savvaidis I, Hughes MN, Poole RK (2003) Copper biosorption by Pseudomonas cepacia and other strains. World J Microbiol Biotechnol 19:117–121

  12. Swaminathan T, Pakshirajan K (2006) Continuous biosorption of Pb, Cu and Cd by bphanerochaete chrysoporium in a packed column reactor. Soil Sediment Contam 15:187–197

  13. Volesky B, Holan ZR (1995) Biosorption of heavy metals. Biotechnol Prog 11:235–250

  14. Wang JL (2002) Technique of organism immobilization and water pollution control. Science Press, Bei Jing (in Chinese)

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This research was supported by Key Science and Technology project of China-Israel Cooperation Fund of Department of Science and Technology, Heilongjiang Province, China (WB07N01); Natural Science Fund Project of Hei Longjiang Province (E2007-16) and National Scientific and Technological Supporting Project(2007BAD87B03).

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Correspondence to Ying Zhang or Qixing Zhou.

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Zhang, Y., Fan, C., Meng, Q. et al. Biosorption of Pb2+ by Saccharomyces Cerevisiae in Static and Dynamic Adsorption Tests. Bull Environ Contam Toxicol 83, 708 (2009).

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  • Saccharomyces cerevisiae
  • Static test
  • Dynamic test
  • Pb2+ adsorption